Montironi R, Galluzzi C M, Diamanti L, Giannulis I, Pisani E, Scarpelli M
Institute of Morbid Anatomy and Histopathology, University of Ancona, Nuovo Ospedale Regionale, Torrette di Ancona, Italy.
Virchows Arch A Pathol Anat Histopathol. 1993;422(3):185-92. doi: 10.1007/BF01621801.
The expression and location of proliferating cell nuclear antigen (PCNA) immunostaining in epithelial, endothelial and stromal nuclei were assessed in prostatic intra-epithelial neoplasia (PIN). It was then compared with patterns in benign lesions and in invasive adenocarcinomas of the prostate. The PCNA-positive nuclei showed homogeneous or granular types of staining, or a mixture of both, and a gradation in the intensity of staining. Nuclei with granular and mixed patterns appeared lighter brown than those with a homogeneous pattern, which are darker and more often noted in PIN and invasive adenocarcinomas than in benign lesions. For epithelial PCNA-stained nuclei, the proportions in the two grades of PIN were greater than in benign prostatic hyperplasia (mean 3.16%, SE 0.31%) and prostatic atrophic ducts and acini (mean 0.56%, SE 0.09%), the values decreasing from the nuclei in the basal position towards those in the luminal layer. In grade 1, the category mean values were 9.51% (SE 1.14%) in the basal, 7.02% (SE 1.27%) in the intermediate and 6.02% (SE 0.90%) in the luminal position. In grade 2, the category mean values were 13.81% (SE 1.42%) in the basal position, 10.99% (SE 1.17%) in the intermediate and 7.91% (SE 1.43%) in the luminal position. In small and large acinar adenocarcinomas, the proportions of positive nuclei were 8.66% (SE 0.30%) and 9.06% (SE 0.30%), respectively. The category mean values in the cribriform adenocarcinomas were 14.40% (SE 0.61%) in the basal position, 11.84% (SE 1.30%) in the intermediate and 9.26% (SE 0.66%) in the luminal position. As in PIN, the proportions of immunostained nuclei in the adenocarcinoma with cribriform pattern decreased from the basal towards the luminal layer. In the solid/trabecular adenocarcinomas, the category mean value in the cell layer adjacent to the stroma was 17.60% (SE 2.92%), whereas in the other cell layers it was lower than that in the cells adjacent the stroma (mean 13.88%, SE 1.71%). For capillary endothelial and stromal cells, the percentages of PCNA-stained nuclei were much lower than those in the epithelial component. The lowest mean values were obtained in benign lesions, whereas the highest were in invasive adenocarcinomas, the percentages in PIN being intermediate.
在前列腺上皮内瘤变（PIN）中评估增殖细胞核抗原（PCNA）免疫染色在上皮细胞核、内皮细胞核和基质细胞核中的表达及定位。然后将其与前列腺良性病变和浸润性腺癌中的模式进行比较。PCNA阳性细胞核呈现均匀或颗粒状染色类型，或两者混合，且染色强度有梯度变化。具有颗粒状和混合模式的细胞核比具有均匀模式的细胞核颜色浅，均匀模式的细胞核颜色更深，且在PIN和浸润性腺癌中比在良性病变中更常见。对于上皮PCNA染色的细胞核，PIN两级中的比例高于良性前列腺增生（平均3.16%，标准误0.31%）和前列腺萎缩性导管及腺泡（平均0.56%，标准误0.09%），从基底位置的细胞核到管腔层的细胞核，该值逐渐降低。在1级中，基底类别平均值为9.51%（标准误1.14%），中间为7.02%（标准误1.27%），管腔位置为6.02%（标准误0.90%）。在2级中，基底位置类别平均值为13.81%（标准误1.42%），中间为10.99%（标准误1.17%），管腔位置为7.91%（标准误1.43%）。在小腺泡腺癌和大腺泡腺癌中，阳性细胞核的比例分别为8.66%（标准误0.30%）和9.06%（标准误0.30%）。筛状腺癌中各类别平均值在基底位置为14.40%（标准误0.61%），中间为11.84%（标准误1.30%），管腔位置为9.26%（标准误0.66%）。与PIN一样，筛状模式腺癌中免疫染色细胞核的比例从基底到管腔层逐渐降低。在实性/小梁状腺癌中，与基质相邻的细胞层类别平均值为17.60%（标准误2.92%），而在其他细胞层低于与基质相邻的细胞（平均13.88%，标准误1.71%）。对于毛细血管内皮细胞和基质细胞，PCNA染色细胞核的百分比远低于上皮成分中的百分比。良性病变中获得的平均值最低，而浸润性腺癌中最高，PIN中的百分比介于两者之间。
