Falini B, Flenghi L, Pileri S, Gambacorta M, Bigerna B, Durkop H, Eitelbach F, Thiele J, Pacini R, Cavaliere A
Institute of Hematology, Perugia University, Italy.
Am J Pathol. 1993 May;142(5):1359-72.
A new anti-macrophage monoclonal antibody (PG-M1) was produced by immunizing BALB/c mice with fresh spleen cells from a patient with Gaucher's disease. PG-M1 reacts strongly with a fixative-resistant epitope of an intracytoplasmic molecule, selectively expressed by virtually all macrophages of the human body. Although attempts to immunoprecipitate the molecule recognized by PG-M1 have failed so far, the reactivity of the antibody with COS-1 and WOP cells transfected with a human complementary DNA clone encoding for the CD68 antigen suggests that PG-M1 is a new member of the CD68 cluster. However, unlike other CD68 antibodies (KP1, EBM11, etc.), which react with both macrophages and myeloid cells, PG-M1 detects a fixative-resistant epitope on the macrophage-restricted form of the CD68 antigen. In 957 routinely fixed, paraffin-embedded samples, PG-M1 showed a more restricted reactivity with elements of the monocyte/macrophage lineage than the previously described monoclonal antibodies MAC-387 (anti-calgranulins), KP1 (CD68) and Ki-M1P. Among hematological malignancies, PG-M1 only labels acute leukemias of M4 and M5 type and rare examples of malignant histiocytosis/true histiocytic sarcoma. In contrast, acute leukemias of the M1, M2, M3, M6, M7, and L1-L3 types, non-Hodgkin's lymphomas, and Hodgkin and Reed-Sternberg cells of Hodgkin's disease are consistently PG-M1-negative. In the daily diagnostic practice, PG-M1 seems to be particularly valuable for the diagnosis of myelomonocytic or monocytic leukemia and neoplasms of true histiocytic origin in routine paraffin sections.
用一名戈谢病患者的新鲜脾细胞免疫BALB/c小鼠，制备出一种新的抗巨噬细胞单克隆抗体（PG-M1）。PG-M1与一种胞质内分子的抗固定剂表位强烈反应，该表位由人体几乎所有巨噬细胞选择性表达。尽管目前试图免疫沉淀PG-M1识别的分子未成功，但该抗体与转染了编码CD68抗原的人互补DNA克隆的COS-1和WOP细胞的反应性表明，PG-M1是CD68簇的一个新成员。然而，与其他既与巨噬细胞又与髓样细胞反应的CD68抗体（KP1、EBM11等）不同，PG-M1检测到CD68抗原巨噬细胞限制性形式上的一个抗固定剂表位。在957份常规固定、石蜡包埋的样本中，与先前描述的单克隆抗体MAC-387（抗钙粒蛋白）、KP1（CD68）和Ki-M1P相比，PG-M1对单核细胞/巨噬细胞系成分的反应性更具限制性。在血液系统恶性肿瘤中，PG-M1仅标记M4和M5型急性白血病以及罕见的恶性组织细胞增多症/真性组织细胞肉瘤病例。相比之下，M1、M2、M3、M6、M7和L1-L3型急性白血病、非霍奇金淋巴瘤以及霍奇金病的霍奇金和里德-斯腾伯格细胞始终为PG-M1阴性。在日常诊断实践中，PG-M1对于常规石蜡切片中髓单核细胞或单核细胞白血病以及真性组织细胞起源肿瘤的诊断似乎特别有价值。
