Yamaguchi H, Hirai S, Shoji M, Harigaya Y, Okamoto Y, Nakazato Y
College of Medical Care and Technology, School of Medicine, Gunma University, Japan.
Prog Clin Biol Res. 1989;317:467-74.
We classified senile plaques (SP) into 4 types: diffuse, primitive, classic, and compact. Here, we studied the nature of diffuse plaques in the brains of 6 autopsied subjects with senile dementia of the Alzheimer type (SDAT). In the entorhinal cortex, both the modified Bielschowsky stain (108%) and periodic acid-methenamine silver (PAM) stain (111%) revealed a number of SP similar to that labeled by beta protein immunostain (100% as standard). The Bodian stain, however, labeled only a minor proportion (37%) of SP. The vast majority of Bodian-negative plaques, which were devoid of swollen neurites, were diffuse plaques. They were seen as ill-defined areas of fine fibrillar materials. Diffuse plaques were argyrophilic when labeled by the modified Bielschowsky and PAM stains. However, they became undetectable when sections were pretreated with formic acid. Formic acid pretreatment made the diffuse plaques immunoreactive with beta protein antiserum. Alzheimer's neurofibrillary tangles (ANT) and the neuritic components of the primitive and classic plaques remained argyrophilic even after pretreatment with formic acid. Our findings suggest that formic acid pretreatment selectively abolishes the argyrophilia of the amyloid-related component of SP, and the diffuse plaques consist mainly of amyloid-related, while the neuritic component does not. Positive reaction products for beta protein were scattered in the SP by immunoelectron microscopy. Some of the reaction products labeled 10 nm filaments resembling amyloid fibrils.
我们将老年斑（SP）分为4种类型：弥漫型、原始型、经典型和致密型。在此，我们研究了6例阿尔茨海默型老年性痴呆（SDAT）尸检对象大脑中弥漫型斑块的性质。在内嗅皮质，改良的 Bielschowsky 染色（108%）和高碘酸-亚甲胺银（PAM）染色（111%）显示的SP数量与β蛋白免疫染色标记的数量相似（以100%为标准）。然而，Bodian 染色仅标记了一小部分（37%）的SP。绝大多数Bodian 阴性斑块没有肿胀的神经突，属于弥漫型斑块。它们表现为细纤维物质的边界不清的区域。当用改良的 Bielschowsky 和 PAM 染色标记时，弥漫型斑块呈嗜银性。然而，当切片用甲酸预处理后，它们就检测不到了。甲酸预处理使弥漫型斑块与β蛋白抗血清产生免疫反应。即使在用甲酸预处理后，阿尔茨海默神经原纤维缠结（ANT）以及原始型和经典型斑块的神经突成分仍保持嗜银性。我们的研究结果表明，甲酸预处理选择性地消除了SP中淀粉样蛋白相关成分的嗜银性，弥漫型斑块主要由淀粉样蛋白相关成分组成，而神经突成分则不然。通过免疫电子显微镜观察，β蛋白的阳性反应产物散在于SP中。一些反应产物标记了类似于淀粉样纤维的10 nm细丝。
