胆碱能和五肽胃泌素对清醒家兔胃酸、胃蛋白酶和脂肪酶分泌的刺激作用。

Cholinergic and pentagastrin stimulation of the gastric secretions of acid, pepsin and lipase in the awake rabbit.

作者信息

Perret J P, Daoud-Elbaba M, Rakotomalala H, Gros I, Descroix-Vagne M

机构信息

Laboratoire de Physiologie de la Nutrition, I.U.T.A., Villeurbanne, France.

出版信息

Arch Int Physiol Biochim Biophys. 1993 Jan-Feb;101(1):87-91. doi: 10.3109/13813459308998135.

DOI:10.3109/13813459308998135

PMID:7684285

Abstract

Acid, pepsin and lipase secretions were simultaneously studied in awake rabbits with a Heidenhain pouch during cholinergic (carbamylcholine 20 micrograms.kg-1 x h-1) or maximal pentagastrin stimulation (64 mu.kg-1 x h-1). To avoid lipase inactivation by low pH, the pouch was perfused at a constant pH, with a solution irrigating the pouch without inducing any pressure. Acid and pepsin outputs were equally activated by both stimulants. Lipase output was stimulated much more by pentagastrin (x26) than by carbamylcholine (x6). The maximal lipase output preceded that of acid output. Lipase concentration increased in the gastric juice whereas pepsin concentration remained constant. A negative correlation was calculated between acid and lipase or pepsin and lipase output. A positive correlation was obtained between acid and pepsin. Our data show that pepsin and lipase secretions which come from distinct chief cells, in the rabbit, responded to the same stimulants but with quantitative and timing differences.

摘要

在清醒的家兔中，利用海登海因小胃同时研究了在胆碱能（氨甲酰胆碱20微克·千克⁻¹·小时⁻¹）或最大五肽胃泌素刺激（64微克·千克⁻¹·小时⁻¹）下的胃酸、胃蛋白酶和脂肪酶分泌情况。为避免低pH导致脂肪酶失活，以恒定pH灌注小胃，所用溶液对小胃进行冲洗且不产生任何压力。两种刺激物对胃酸和胃蛋白酶的分泌均有同等程度的激活作用。五肽胃泌素对脂肪酶分泌的刺激作用（增至26倍）远大于氨甲酰胆碱（增至6倍）。脂肪酶的最大分泌量先于胃酸分泌量出现。胃液中脂肪酶浓度升高，而胃蛋白酶浓度保持恒定。计算得出胃酸与脂肪酶或胃蛋白酶与脂肪酶分泌量之间呈负相关。胃酸与胃蛋白酶之间呈正相关。我们的数据表明，家兔中源自不同主细胞的胃蛋白酶和脂肪酶分泌对相同刺激物有反应，但在数量和时间上存在差异。