Gray K D, Roth M J
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
J Virol. 1993 Jun;67(6):3489-96. doi: 10.1128/JVI.67.6.3489-3496.1993.
The env gene products of Moloney murine leukemia virus are required for binding and entry of the virus into the target cell. Thirty-three linker insertion mutations were constructed throughout the env gene of Moloney murine leukemia virus. Twenty of the mutations were located in the surface protein (SU), and the remaining thirteen were located in the transmembrane protein (TM). The viability of the viruses containing these env gene mutations was determined by performing transient transfections and screening for the release of reverse transcriptase. Eleven viable mutants were isolated, nine in SU and two in TM. Three of the viable mutants were temperature sensitive. Four of the viable mutants were clustered in the carboxy terminus of SU. The env gene products of transfected cell lines which produced viable virus were analyzed. Our results indicated two regions of SU important for the stability of the SU/TM heteropolymer and one region important for the interaction of the env gene products with the viral core.
莫洛尼鼠白血病病毒的env基因产物是病毒与靶细胞结合及进入所必需的。在莫洛尼鼠白血病病毒的整个env基因中构建了33个接头插入突变。其中20个突变位于表面蛋白（SU），其余13个位于跨膜蛋白（TM）。通过进行瞬时转染并筛选逆转录酶的释放来确定含有这些env基因突变的病毒的活力。分离出11个活的突变体，9个在SU中，2个在TM中。3个活的突变体对温度敏感。4个活的突变体聚集在SU的羧基末端。对产生活病毒的转染细胞系的env基因产物进行了分析。我们的结果表明，SU有两个区域对SU/TM异聚体的稳定性很重要，还有一个区域对env基因产物与病毒核心的相互作用很重要。
