Guzman J, Arbogast S, Bross K J, Finke R, Costabel U
University of Bochum, Germany.
Acta Cytol. 1993 May-Jun;37(3):267-71.
It is not known how long cell surface antigens can be detected on lymphocytes in pleural effusions. Therefore, the lymphocyte subpopulations of 15 native pleural effusions were analyzed after different storage times, at either 4 degrees C or room temperature, using the peroxidase-antiperoxidase adhesive slide assay. No significant differences in the lymphocyte subpopulations were observed after one day of storage under both conditions, although the immunoreactivity with CD4 was poor in the majority of cases stored at 4 degrees C and in two cases stored at room temperature. After three days of storage at 4 degrees C and after four days of storage at room temperature, a marked decrease in lymphocytes attached to the slides was observed. Immunoreactivity with CD8, CD20, CD45 and HLA-1 was well preserved, also, after one week of storage. Reactivity with CD3 was weak or poor after three days of storage in some cases. It is important to recognize that the preservation of the immunoreactivity of lymphocytes is dependent not only on the nutritive quality of pleural fluids but also on the cell preparation method.
目前尚不清楚胸腔积液中淋巴细胞表面抗原能够被检测到的时长。因此，运用过氧化物酶 - 抗过氧化物酶黏附玻片检测法，对15份天然胸腔积液在4℃或室温下经过不同储存时间后的淋巴细胞亚群进行了分析。在两种条件下储存一天后，未观察到淋巴细胞亚群有显著差异，不过在多数4℃储存的样本以及两份室温储存的样本中，与CD4的免疫反应性较差。在4℃储存三天以及室温储存四天后，观察到附着在玻片上的淋巴细胞显著减少。在储存一周后，与CD8、CD20、CD45和HLA - 1的免疫反应性也保存良好。在某些情况下，储存三天后与CD3的反应性较弱或较差。必须认识到，淋巴细胞免疫反应性的保存不仅取决于胸腔积液的营养质量，还取决于细胞制备方法。
