Guzman J, Bross K J, Costabel U
Department of Pathology, University of Freiburg, Federal Republic of Germany.
Acta Cytol. 1990 Jul-Aug;34(4):497-501.
Thirteen malignant pleural effusions due to small cell carcinoma (SCC) of the lung were immunocytochemically studied using the peroxidase-antiperoxidase adhesive slide assay for the determination of cell surface antigens. A panel of monoclonal antibodies (MAbs) was used to determine the lymphocyte subpopulations and the reactivity of the tumor cells. Of the lymphocytes, 87 +/- 1% were CD3+ T cells, with 72 +/- 10% CD4+ helper/inducer T cells and 20 +/- 5% CD8+ suppressor/cytotoxic T cells. Only a minority of T lymphocytes were activated in terms of expressing the surface markers CD38 and HLA-DR. The distribution of the lymphocyte subpopulations was not significantly different from the distribution in other malignant and nonmalignant pleural diseases previously studied, indicating that the reaction pattern of the lymphocytes in the pleural cavity is similar in different diseases. The tumor cells from all cases were positive for LeuM1, CD16 and HLA-DR; 10 of 11 cases were positive for HEA-125, Sam 2 and Sam 10. Positivity for epithelial membrane antigen was observed in 11 cases, for OKT9 in 8 cases and for carcinoembryonic antigen in 6 cases. A total or partial loss of the reactivity with HLA-1 was found in nine cases. The reactivity pattern of the tumor cells with the MAbs used in this study is not specific for SCC of the lung because other carcinoma cells also reacted with these markers. Additional morphologic criteria, such as cell size and cell configuration, are needed to recognize the immunocytochemically positive-reacting cells as tumor cells from SCC of the lung. However, the immunostaining allows a better identification of the tumor cells, especially in cases with a small quantity of tumor cells.
应用过氧化物酶 - 抗过氧化物酶黏附玻片法对13例因肺小细胞癌（SCC）所致的恶性胸腔积液进行免疫细胞化学研究，以测定细胞表面抗原。使用一组单克隆抗体（MAbs）来确定淋巴细胞亚群以及肿瘤细胞的反应性。在淋巴细胞中，87±1%为CD3⁺ T细胞，其中72±10%为CD4⁺辅助/诱导性T细胞，20±5%为CD8⁺抑制/细胞毒性T细胞。就表达表面标志物CD38和HLA - DR而言，只有少数T淋巴细胞被激活。淋巴细胞亚群的分布与先前研究的其他恶性和非恶性胸腔疾病中的分布无显著差异，这表明不同疾病中胸腔内淋巴细胞的反应模式相似。所有病例的肿瘤细胞对LeuM1、CD16和HLA - DR呈阳性；在11例中有10例对HEA - 125、Sam 2和Sam 10呈阳性。11例观察到上皮膜抗原呈阳性，8例对OKT9呈阳性，6例对癌胚抗原呈阳性。9例发现与HLA - 1的反应性全部或部分丧失。本研究中所用MAbs与肿瘤细胞的反应模式并非肺SCC所特有，因为其他癌细胞也与这些标志物发生反应。需要额外的形态学标准，如细胞大小和细胞形态，来将免疫细胞化学阳性反应细胞识别为肺SCC的肿瘤细胞。然而，免疫染色能更好地识别肿瘤细胞，尤其是在肿瘤细胞数量较少的病例中。
