从副溶血性弧菌一种新的O抗原形式（O13）中分离出的脂多糖。

Lipopolysaccharide isolated from a new O-antigenic form (O13) of Vibrio parahaemolyticus.

作者信息

Hisatsune K, Iguchi T, Haishima Y, Tamura N, Kondo S

机构信息

Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.

出版信息

Microbiol Immunol. 1993;37(2):143-7. doi: 10.1111/j.1348-0421.1993.tb03191.x.

DOI:10.1111/j.1348-0421.1993.tb03191.x

PMID:7684809

Abstract

The chemical properties of a lipopolysaccharide (LPS) isolated from a new O-antigenic form (O13) of Vibrio parahaemolyticus were investigated. The LPS contained glucose, galactose, L-glycero-D-manno-heptose and glucosamine. 2-Keto-3-deoxy-octonate (KDO) was not detected in the LPS by the periodate-thiobarbituric acid test (Weissbach's reaction) under conventional hydrolysis conditions. Instead, phosphorylated KDO (X1 and X2) was found in its strong-acid hydrolysate. This sugar composition was identical to that of V. parahaemolyticus O3, O5 and O11 LPS, indicating that, based on the sugar composition, O13 LPS belongs to Chemotype III to which O3, O5 and O11 belong. In addition, structural study demonstrated the presence of KDO 4-phosphate in its inner-core region.

摘要

对从副溶血性弧菌新的O抗原型（O13）分离出的脂多糖（LPS）的化学性质进行了研究。该LPS含有葡萄糖、半乳糖、L-甘油-D-甘露庚糖和葡糖胺。在常规水解条件下，通过高碘酸-硫代巴比妥酸试验（魏斯巴赫反应）在LPS中未检测到2-酮-3-脱氧辛酸（KDO）。相反，在其强酸水解产物中发现了磷酸化的KDO（X1和X2）。这种糖组成与副溶血性弧菌O3、O5和O11 LPS的糖组成相同，表明基于糖组成，O13 LPS属于O3、O5和O11所属的化学型III。此外，结构研究表明在其内核区域存在KDO 4-磷酸。