从副溶血性弧菌分离的脂多糖中2-酮-3-脱氧-D-甘露辛酮酸的存在情况。
Occurrence of 2-keto-3-deoxy-D-manno-octonic acid in lipopolysaccharides isolated from Vibrio parahaemolyticus.
作者信息
Han T J, Chai T J
机构信息
Horn Point Environmental Laboratory, University of Maryland, Cambridge 21613.
出版信息
J Bacteriol. 1991 Oct;173(19):6303-6. doi: 10.1128/jb.173.19.6303-6306.1991.
Abstract
The occurrence of 2-keto-3-deoxy-D-manno-octonic acid (KDO) in lipopolysaccharides (LPS) of Vibrio parahaemolyticus was demonstrated for the first time by gas chromatography-mass spectrometry after dephosphorylation, reduction, and methylation. KDO was virtually completely phosphorylated, since no KDO was detected by either gas chromatography or thiobarbituric acid assay before dephosphorylation. The level of KDO in all six strains of V. parahaemolyticus investigated ranged from 0.37 to 0.69%, which was considerably lower than that in enterobacterial LPS.
摘要
通过气相色谱-质谱联用技术，在去磷酸化、还原和甲基化之后，首次证实了副溶血性弧菌脂多糖（LPS）中存在2-酮-3-脱氧-D-甘露辛酮酸（KDO）。KDO几乎完全磷酸化，因为在去磷酸化之前，通过气相色谱或硫代巴比妥酸测定均未检测到KDO。在所研究的所有六株副溶血性弧菌中，KDO的含量在0.37%至0.69%之间，这大大低于肠杆菌LPS中的含量。
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1
2
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Microbiol Immunol. 1980;24(8):691-701. doi: 10.1111/j.1348-0421.1980.tb02870.x.
3
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J Bacteriol. 1982 Jul;151(1):367-75. doi: 10.1128/jb.151.1.367-375.1982.
4
2-Amino-2,6-dideoxy-d-glucose (D-quinovosamine): a constituent of the lipopolysaccharides of Vibrio cholerae.
Eur J Biochem. 1973 Sep 3;37(3):531-4. doi: 10.1111/j.1432-1033.1973.tb03015.x.
5
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6
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Biochem Biophys Res Commun. 1987 Mar 30;143(3):845-7. doi: 10.1016/0006-291x(87)90326-3.
7
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Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Apr;268(2):151-79. doi: 10.1016/s0176-6724(88)80001-4.
8
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Epidemiol Infect. 1989 Aug;103(1):1-34. doi: 10.1017/s0950268800030326.
9
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10
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