与化学核酸酶相连的RNA对DNA进行序列特异性切割。
Sequence-specific scission of DNA by RNAs linked to a chemical nuclease.
作者信息
Sigman D S, Chen C H, Gorin M B
机构信息
Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024-1570.
出版信息
Nature. 1993 Jun 3;363(6428):474-5. doi: 10.1038/363474a0.
DOI:10.1038/363474a0
PMID:7684825
Abstract
RNAs linked to the chemical nuclease 1,10-phenanthroline-copper cut double-stranded DNA of complementary sequence. This cleavage reaction is applicable to all sequences and can be used to measure the distance between marker genes in base pairs, map the size of a transcription unit and define positions of chromosomal breakpoints.
摘要
与化学核酸酶1,10 - 菲咯啉 - 铜相关的RNA可切割互补序列的双链DNA。这种切割反应适用于所有序列，可用于以碱基对为单位测量标记基因之间的距离、绘制转录单位的大小以及确定染色体断点的位置。
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