用于体外筛选的修饰RNA序列库。
Modified RNA sequence pools for in vitro selection.
作者信息
Lin Y, Qiu Q, Gill S C, Jayasena S D
机构信息
NeXagen, Inc., Boulder, CO 80301.
出版信息
Nucleic Acids Res. 1994 Dec 11;22(24):5229-34. doi: 10.1093/nar/22.24.5229.
We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.
我们报道了一种修饰RNA的应用，其中嘧啶的2'-羟基被2'-氨基（2'-NH2）取代，通过体外筛选来鉴定对人中性粒细胞弹性蛋白酶（HNE）具有特异性的高亲和力配体。与未修饰的RNA相比，2'-NH2修饰的RNA配体在人血清和尿液中表现出更高的稳定性。在K+和Li+离子存在下使用核糖核酸酶T1切割数据表明，为HNE选择的修饰RNA配体形成了分子间G-四联体结构。
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