Palmer R M, Hickery M S, Charles I G, Moncada S, Bayliss M T
Wellcome Research Laboratories, Beckenham, Kent, UK.
Biochem Biophys Res Commun. 1993 May 28;193(1):398-405. doi: 10.1006/bbrc.1993.1637.
Incubation of human chondrocytes with interleukin-1 beta, tumour necrosis factor or endotoxin induced the expression of NO synthase. The synthesis of NO induced by IL-1 beta was concentration- and time- dependent, occurred after a lag period of approximately 6h and was inhibited by NG-monomethyl-L-arginine, cycloheximide, dexamethasone and hydrocortisone, but not by indomethacin. The activity of NO synthase from activated chondrocytes was not affected by EGTA or by the calmodulin inhibitor W-13. Northern blot analysis, with a rabbit chondrocyte inos probe, showed a 4.4kb positively hybridising band from activated human chondrocytes. Thus, human articular chondrocytes express an inducible NO synthase from the same family as the rabbit chondrocyte and rodent macrophage enzymes. This family appears to vary in terms of in vitro Ca(2+)-dependence and sensitivity to glucocorticoids.
用人白细胞介素-1β、肿瘤坏死因子或内毒素孵育人软骨细胞可诱导一氧化氮合酶的表达。白细胞介素-1β诱导的一氧化氮合成呈浓度和时间依赖性，在约6小时的延迟期后出现，并被NG-单甲基-L-精氨酸、放线菌酮、地塞米松和氢化可的松抑制，但不受吲哚美辛抑制。活化软骨细胞中一氧化氮合酶的活性不受乙二醇双四乙酸（EGTA）或钙调蛋白抑制剂W-13的影响。用兔软骨细胞肌醇探针进行的Northern印迹分析显示，活化的人软骨细胞有一条4.4kb的阳性杂交带。因此，人关节软骨细胞表达一种与兔软骨细胞和啮齿动物巨噬细胞酶同属一个家族的诱导型一氧化氮合酶。该家族在体外对钙离子的依赖性和对糖皮质激素的敏感性方面似乎有所不同。
