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在DNA、RNA和寡核苷酸类似物合成中对核苷进行不稳定的环外胺保护，有助于N-脱酰化，使脱嘌呤和链降解最小化。

Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation.

作者信息

Sinha N D, Davis P, Usman N, Pérez J, Hodge R, Kremsky J, Casale R

机构信息

Nucleic Acid Chemistry Group, Millipore Corporation, Bedford, MA 01730.

出版信息

Biochimie. 1993;75(1-2):13-23. doi: 10.1016/0300-9084(93)90019-o.

DOI:10.1016/0300-9084(93)90019-o
PMID:7684933
Abstract

The use of tert-butylphenoxyacetyl for N-protection of nucleoside bases during oligonucleotide synthesis facilitates its removal under mild conditions. This protecting group reduces depurination of deoxyadenosine residues, and minimizes premature desilylation resulting in chain degradation during post-synthesis workup of synthetic RNA.

摘要

在寡核苷酸合成过程中，使用叔丁基苯氧基乙酰基对核苷碱基进行N-保护有利于在温和条件下将其去除。该保护基团减少了脱氧腺苷残基的脱嘌呤作用，并使合成RNA合成后处理过程中导致链降解的过早去硅烷化作用降至最低。

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