从可控孔径玻璃载体上切割寡脱氧核糖核苷酸及其通过气态胺进行的快速脱保护。
Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines.
作者信息
Boal J H, Wilk A, Harindranath N, Max E E, Kempe T, Beaucage S L
机构信息
Division of Hematologic Product, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
出版信息
Nucleic Acids Res. 1996 Aug 1;24(15):3115-7. doi: 10.1093/nar/24.15.3115.
Abstract
A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tert-butyl)phenoxyacetyl group for the protection of the exocyclic amino function of cytosine, adenine and guanine were released from controlled-pore glass supports and fully deprotected by ammonia or methylamine under gas phase conditions, at room temperature, within 35 or 2 min respectively.
摘要
已开发出一种用于寡脱氧核糖核苷酸脱保护的新方法。在压力下使用气态胺，如氨或甲胺，以实现温和且快速的脱保护条件。例如，具有（叔丁基）苯氧基乙酰基用于保护胞嘧啶、腺嘌呤和鸟嘌呤的环外氨基功能的寡脱氧核糖核苷酸，从可控孔径玻璃载体上释放出来，并在室温下分别于35分钟或2分钟内在气相条件下用氨或甲胺完全脱保护。
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Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation.在DNA、RNA和寡核苷酸类似物合成中对核苷进行不稳定的环外胺保护，有助于N-脱酰化，使脱嘌呤和链降解最小化。
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