• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines.从可控孔径玻璃载体上切割寡脱氧核糖核苷酸及其通过气态胺进行的快速脱保护。
Nucleic Acids Res. 1996 Aug 1;24(15):3115-7. doi: 10.1093/nar/24.15.3115.
2
Microwave-assisted rapid deprotection of oligodeoxyribonucleotides.微波辅助寡脱氧核糖核苷酸的快速脱保护
Nucleic Acids Res. 1997 Dec 15;25(24):5127-9. doi: 10.1093/nar/25.24.5127.
3
A simple method for N-15 labelling of exocyclic amino groups in synthetic oligodeoxynucleotides.一种用于对合成寡脱氧核苷酸中环外氨基进行N-15标记的简单方法。
Nucleic Acids Res. 1994 Aug 11;22(15):2982-9. doi: 10.1093/nar/22.15.2982.
4
The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups.通过使用不稳定的碱基保护基团，寡核苷酸合成中的最终脱保护步骤简化为温和且快速的氨处理。
Nucleic Acids Res. 1987 Jan 26;15(2):397-416. doi: 10.1093/nar/15.2.397.
5
Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid-phase supports.用于将第一个核苷连接到未衍生化固相载体上的连接子亚磷酰胺试剂。
Nucleic Acids Res. 2004 Jan 29;32(2):623-31. doi: 10.1093/nar/gkh222. Print 2004.
6
A simple method for the solid phase synthesis of oligodeoxynucleotides containing O4-alkylthymine.一种用于固相合成含O4-烷基胸腺嘧啶的寡脱氧核苷酸的简单方法。
Nucleic Acids Res. 1990 Jul 25;18(14):4061-5. doi: 10.1093/nar/18.14.4061.
7
Rapid conditions for the cleavage of oligodeoxyribonucleotides from cis-diol-bearing universal polymer supports and their deprotection.从含顺式二醇的通用聚合物载体上切割寡脱氧核糖核苷酸的快速条件及其脱保护。
Nucleic Acids Res. 1999 May 15;27(10):e2. doi: 10.1093/nar/27.10.e2.
8
[Directed introduction of amino groups by internucleotide phosphate group during solid-phase synthesis of oligodeoxyribonucleotides. Preparation of biotinylated oligonucleotide probes].[寡脱氧核糖核苷酸固相合成过程中通过核苷酸间磷酸基团定向引入氨基。生物素化寡核苷酸探针的制备]
Bioorg Khim. 1991 May;17(5):685-9.
9
Automated synthesis of oligodeoxyribonucleoside methylphosphonates having [N-(3-aminoprop-1-yl)-N-(2-hydroxyethyl)-2-aminoethyl] phosphate or methylphosphonic acid at the 3' end using a modified controlled pore glass support.使用改性的可控孔径玻璃载体自动合成在3'端具有[ N-（3-氨基丙-1-基）-N-（2-羟乙基）-2-氨基乙基]磷酸酯或甲基膦酸的寡脱氧核糖核苷甲基膦酸酯。
Bioconjug Chem. 1993 Sep-Oct;4(5):395-401. doi: 10.1021/bc00023a015.
10
The application of the AMB protective group in the solid-phase synthesis of methylphosphonate DNA analogues.AMB保护基团在甲基膦酸酯DNA类似物固相合成中的应用。
Nucleic Acids Res. 1993 Jul 25;21(15):3493-500. doi: 10.1093/nar/21.15.3493.

引用本文的文献

1
Gas-phase cleavage and dephosphorylation of universal linker-bound oligodeoxynucleotides.通用接头连接的寡脱氧核苷酸的气相裂解和去磷酸化
Nucleosides Nucleotides Nucleic Acids. 2010 Nov;29(11):867-78. doi: 10.1080/15257770.2010.534757.
2
Synthesis of oligodeoxynucleotides using fully protected deoxynucleoside 3'-phosphoramidite building blocks and base recognition of oligodeoxynucleotides incorporating N3-cyano-ethylthymine.使用全保护的脱氧核苷 3′-亚磷酰胺基构筑块合成寡脱氧核苷酸，以及识别含 N3-氰乙基胸腺嘧啶的寡脱氧核苷酸的碱基。
Molecules. 2010 Oct 27;15(11):7509-31. doi: 10.3390/molecules15117509.
3
Derivatization of DNAs with selenium at 6-position of guanine for function and crystal structure studies.用于功能和晶体结构研究的在鸟嘌呤6位用硒对DNA进行衍生化。
Nucleic Acids Res. 2008 Dec;36(22):7009-18. doi: 10.1093/nar/gkn843. Epub 2008 Nov 5.
4
Thermolytic CpG-containing DNA oligonucleotides as potential immunotherapeutic prodrugs.作为潜在免疫治疗前体药物的热解含CpG DNA寡核苷酸
Nucleic Acids Res. 2005 Jun 21;33(11):3550-60. doi: 10.1093/nar/gki657. Print 2005.
5
High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer.使用1536通道合成仪进行寡核苷酸的高通量平行合成。
Nucleic Acids Res. 2002 Sep 15;30(18):e93. doi: 10.1093/nar/gnf092.
6
Microwave-assisted rapid deprotection of oligodeoxyribonucleotides.微波辅助寡脱氧核糖核苷酸的快速脱保护
Nucleic Acids Res. 1997 Dec 15;25(24):5127-9. doi: 10.1093/nar/25.24.5127.
7
Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.对苯二酚 - O,O'-二乙酸（“Q 连接体”）在固相寡核苷酸合成中作为琥珀酰和草酰连接臂的替代物。
Nucleic Acids Res. 1997 Sep 15;25(18):3629-35. doi: 10.1093/nar/25.18.3629.

本文引用的文献

1
Oligodeoxyribonucleotide phosphotriesters.寡脱氧核糖核苷酸磷酸三酯。
Methods Mol Biol. 1993;20:207-24. doi: 10.1385/0-89603-281-7:207.
2
A survey of nucleic acid services in core laboratories.核心实验室核酸检测服务调查
Biotechniques. 1994 Sep;17(3):526-34.
3
Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation.在DNA、RNA和寡核苷酸类似物合成中对核苷进行不稳定的环外胺保护，有助于N-脱酰化，使脱嘌呤和链降解最小化。
Biochimie. 1993;75(1-2):13-23. doi: 10.1016/0300-9084(93)90019-o.
4
The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups.通过使用不稳定的碱基保护基团，寡核苷酸合成中的最终脱保护步骤简化为温和且快速的氨处理。
Nucleic Acids Res. 1987 Jan 26;15(2):397-416. doi: 10.1093/nar/15.2.397.
5
The isopropoxyacetic group for convenient base protection during solid-support synthesis of oligodeoxyribonucleotides and their triester analogs.异丙氧基乙酰基用于在寡脱氧核糖核苷酸及其三酯类似物的固相合成过程中方便地进行碱基保护。
Nucleic Acids Res. 1989 Jun 26;17(12):4863-71. doi: 10.1093/nar/17.12.4863.

从可控孔径玻璃载体上切割寡脱氧核糖核苷酸及其通过气态胺进行的快速脱保护。

Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines.

作者信息

Boal J H, Wilk A, Harindranath N, Max E E, Kempe T, Beaucage S L

机构信息

Division of Hematologic Product, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.

出版信息

Nucleic Acids Res. 1996 Aug 1;24(15):3115-7. doi: 10.1093/nar/24.15.3115.

DOI:10.1093/nar/24.15.3115
PMID:8760903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146024/
Abstract

A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tert-butyl)phenoxyacetyl group for the protection of the exocyclic amino function of cytosine, adenine and guanine were released from controlled-pore glass supports and fully deprotected by ammonia or methylamine under gas phase conditions, at room temperature, within 35 or 2 min respectively.

摘要

已开发出一种用于寡脱氧核糖核苷酸脱保护的新方法。在压力下使用气态胺，如氨或甲胺，以实现温和且快速的脱保护条件。例如，具有（叔丁基）苯氧基乙酰基用于保护胞嘧啶、腺嘌呤和鸟嘌呤的环外氨基功能的寡脱氧核糖核苷酸，从可控孔径玻璃载体上释放出来，并在室温下分别于35分钟或2分钟内在气相条件下用氨或甲胺完全脱保护。