Sawaki M, Kita E, Mikasa K, Konishi M, Kumimatsu M, Kashiba S, Narita N
Dept. of Internal Medicine II, Nara Medical University, Japan.
Biotherapy. 1993;6(1):51-61. doi: 10.1007/BF01877386.
The therapeutic effect of granulocyte colony-stimulating factor (G-CSF) against intramuscular infection with Pseudomonas aeruginosa in cyclophosphamide (CY)-treated mice was analyzed by measuring plasma levels of amyloid P-component (APC) and proinflammatory cytokine levels. CY (100 mg/kg) treatment of mice significantly suppressed plasma concentrations of APC and tumor-necrosis factor-alpha (TNF-alpha) following infection with P. aeruginosa, in associated with enhanced susceptibility of the treated mice to this bacterium. A 4-day treatment of CY-treated mice with recombinant human G-CSF (rhG-CSF) increased resistance of CY-treated mice, together with the marked restoration of APC and TNF-alpha productions. The capacity to produce interleukin 1-beta and TNF-alpha of peritoneal macrophages and also that to produce IL-6 of spleen cells were significantly enhanced by the in vivo administration of rhG-CSF in CY-treated mice. These results indicate that G-CSF may increase the functions of monocytes/macrophages directly or indirectly in vivo. Therefore, the therapeutic effect of rhG-CSF seems to consist of not only increases in the number and functions of neutrophils but also enhancement of monocyte/macrophage functions.
通过测量血浆中淀粉样蛋白P成分（APC）水平和促炎细胞因子水平，分析了粒细胞集落刺激因子（G-CSF）对环磷酰胺（CY）处理小鼠肌肉注射铜绿假单胞菌感染的治疗效果。用CY（100mg/kg）处理小鼠后，在感染铜绿假单胞菌后，其血浆中APC和肿瘤坏死因子-α（TNF-α）的浓度显著降低，这与处理后小鼠对该细菌的易感性增强有关。用重组人G-CSF（rhG-CSF）对CY处理的小鼠进行为期4天的治疗，可增加CY处理小鼠的抵抗力，同时显著恢复APC和TNF-α的产生。在CY处理的小鼠体内给予rhG-CSF可显著增强腹膜巨噬细胞产生白细胞介素1-β和TNF-α的能力以及脾细胞产生IL-6的能力。这些结果表明，G-CSF可能在体内直接或间接增加单核细胞/巨噬细胞的功能。因此，rhG-CSF的治疗效果似乎不仅包括中性粒细胞数量和功能的增加，还包括单核细胞/巨噬细胞功能的增强。
