Tanaka T, Okamura S, Okada K, Suga A, Shimono N, Ohhara N, Hirota Y, Sawae Y, Niho Y
Cancer Center, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Infect Immun. 1989 Jun;57(6):1792-9. doi: 10.1128/iai.57.6.1792-1799.1989.
The effects of recombinant murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF) against Pseudomonas aeruginosa infection in ICR mice were investigated. Mice were treated with cyclophosphamide (CPA) and were then injected intraperitoneally with rmGM-CSF three times daily, beginning on the day after CPA treatment, for 7 days. The number of peripheral blood leukocytes in both CPA- and rmGM-CSF-treated mice and control CPA-treated mice reached a nadir on day 4, when P. aeruginosa was injected intraperitoneally. The administration of rmGM-CSF significantly increased the proportion of survivors among mice infected with a lethal dose of P. aeruginosa. This effect was further analyzed by monitoring sequential changes in leukocyte count and bacterial growth in various organs. The number of bacteria in the peritoneal cavities, peripheral blood samples, and livers of GM-CSF-treated mice decreased to an undetectable level after a transient increase, and the number was significantly lower than that in control mice. In GM-CSF-treated mice, the neutrophil levels in peripheral blood started to increase 5 days after CPA administration and were consistently higher than those in controls. Furthermore, the neutrophils in GM-CSF-treated mice were more mature morphologically. Thus, the prophylactic effect of rmGM-CSF against P. aeruginosa infection may result from a rapid recovery of myelopoiesis and a partial enhancement of mature neutrophil function.
研究了重组鼠粒细胞-巨噬细胞集落刺激因子（rmGM-CSF）对ICR小鼠铜绿假单胞菌感染的影响。小鼠用环磷酰胺（CPA）处理，然后从CPA处理后的第二天开始，每天腹腔注射rmGM-CSF三次，共7天。在腹腔注射铜绿假单胞菌的第4天，CPA处理组、rmGM-CSF处理组小鼠以及对照CPA处理组小鼠的外周血白细胞数量均降至最低点。rmGM-CSF的给药显著提高了感染致死剂量铜绿假单胞菌小鼠的存活率。通过监测各器官白细胞计数和细菌生长的连续变化对这一效应进行了进一步分析。GM-CSF处理组小鼠腹腔、外周血样本和肝脏中的细菌数量在短暂增加后降至检测不到的水平，且数量显著低于对照小鼠。在GM-CSF处理组小鼠中，外周血中性粒细胞水平在CPA给药后5天开始升高，并一直高于对照组。此外，GM-CSF处理组小鼠的中性粒细胞在形态上更成熟。因此，rmGM-CSF对铜绿假单胞菌感染的预防作用可能源于骨髓造血的快速恢复和成熟中性粒细胞功能的部分增强。
