表皮生长因子及其受体在滋养层细胞恶性转化中的表达降低。

Decreased expression of epidermal growth factor and its receptor in the malignant transformation of trophoblasts.

作者信息

Ladines-Llave C A, Maruo T, Manalo A M, Mochizuki M

机构信息

Department of Obstetrics and Gynecology, Kobe University School of Medicine, Japan.

出版信息

Cancer. 1993 Jun 15;71(12):4118-23. doi: 10.1002/1097-0142(19930615)71:12<4118::aid-cncr2820711251>3.0.co;2-h.

DOI:10.1002/1097-0142(19930615)71:12<4118::aid-cncr2820711251>3.0.co;2-h

PMID:7685239

Abstract

BACKGROUND

Gestational trophoblastic neoplasms comprise the neoplastic spectrum of nonmalignant hydatidiform mole, invasive hydatidiform mole, and truly malignant choriocarcinoma. Increasing evidence indicates that epidermal growth factor (EGF) acts as an enhancer of trophoblast function to produce human chorionic gonadotropin and that EGF and its receptor may provide a growth advantage to certain carcinoma cells. The current study was undertaken to evaluate a possible link between malignant transformation of trophoblast and expression of EGF and EGF receptor.

METHODS

Cytologic localization and cellular levels of expression of EGF and EGF receptor in hydatidiform mole, invasive hydatidiform mole, and choriocarcinoma tissue specimens were examined by the avidin-biotin immunoperoxidase techniques with monoclonal antibodies against EGF and EGF receptor.

RESULTS

EGF in hydatidiform mole and invasive mole was localized in syncytiotrophoblasts, whereas cytologic localization of EGF receptor in hydatidiform mole and invasive mole was observed in both cytotrophoblasts and syncytiotrophoblasts. By contrast, EGF and EGF receptor in choriocarcinoma were exhibited in cytotrophoblastic and syncytiotrophoblastic elements. Most (72%) hydatidiform moles immunostained intensely for EGF and EGF receptor, whereas most (78%) choriocarcinomas immunostained slightly for EGF and EGF receptor. Invasive mole occupied the middle position in the staining intensity for EGF and EGF receptor, between hydatidiform mole and choriocarcinoma, with 50% of the cases exhibiting moderate staining.

CONCLUSIONS

The simultaneous expression of EGF and EGF receptor in the neoplastic trophoblasts implies that EGF may act in an autocrine-paracrine manner in trophoblastic neoplasms. Furthermore, the results obtained suggest that cytologic expression of EGF and EGF receptor in trophoblastic neoplasms decreases in the malignant transformation of trophoblast.

摘要

背景

妊娠滋养细胞肿瘤包括非恶性葡萄胎、侵蚀性葡萄胎和真正恶性的绒毛膜癌的肿瘤谱系。越来越多的证据表明，表皮生长因子（EGF）作为滋养层细胞功能的增强剂，可产生人绒毛膜促性腺激素，并且EGF及其受体可能为某些癌细胞提供生长优势。本研究旨在评估滋养层细胞恶性转化与EGF和EGF受体表达之间的可能联系。

方法

采用抗EGF和EGF受体的单克隆抗体，通过抗生物素蛋白-生物素免疫过氧化物酶技术，检测葡萄胎、侵蚀性葡萄胎和绒毛膜癌组织标本中EGF和EGF受体的细胞学定位及细胞水平表达。

结果

葡萄胎和侵蚀性葡萄胎中的EGF定位于合体滋养层细胞，而葡萄胎和侵蚀性葡萄胎中EGF受体的细胞学定位在细胞滋养层细胞和合体滋养层细胞中均有观察到。相比之下，绒毛膜癌中的EGF和EGF受体在细胞滋养层细胞和合体滋养层细胞成分中均有表现。大多数（72%）葡萄胎对EGF和EGF受体免疫染色强烈，而大多数（78%）绒毛膜癌对EGF和EGF受体免疫染色较弱。侵蚀性葡萄胎在EGF和EGF受体染色强度方面处于葡萄胎和绒毛膜癌之间的中间位置，50%的病例表现为中度染色。