Janda W M, Wilcoski L M, Mandel K L, Ruther P, Stevens J M
Department of Pathology, University of Illinois, Chicago 60612.
Eur J Clin Microbiol Infect Dis. 1993 Mar;12(3):177-84. doi: 10.1007/BF01967108.
Recently, a chemiluminescent nucleic acid probe test that specifically detects the ribosomal ribonucleic acid of Neisseria gonorrhoeae has been released for clinical laboratory use (AccuProbe Neisseria gonorrhoeae). In this study, three coagglutination tests (GonoGen I, Meritec GC, and GC Omni), the GonoGen II immunofiltration method and the Micro Trak Neisseria gonorrhoeae fluorescent monoclonal antibody test were compared with AccuProbe for identification of gonococci. Strains tested (n = 376) included 194 Neisseria gonorrhoeae, 82 Neisseria meningitidis, 32 Neisseria lactamica, 32 Neisseria species, 32 Moraxella catarrhalis, 2 Moraxella spp. and 2 Kingella denitrificans. The GonoGen I, Meritec GC and GC Omni coagglutination tests produced clearly positive results for 93.8%, 92.3% and 95.9% of the gonococci, respectively. The GonoGen II unequivocally identified 91.8% and the MicroTrak fluorescent antibody test identified 90.7% with 2+ or greater fluorescence. AccuProbe identified 100% of the gonococci tested. GonoGen I and GonoGen II were 98% specific, Meritec GC was 99% specific and the specificity of the GC Omni, MicroTrak fluorescent antibody and AccuProbe tests was 100%. While antibody-based tests were reliable when results were clearly interpretable, the AccuProbe was the only confirmatory test that was 100% accurate. Serotyping studies indicate that an array of beta-lactamase positive and negative gonococcal serotypes fail to react with the monoclonal antibody-based tests in general and with the fluorescent antibody test in particular.
最近，一种专门检测淋病奈瑟菌核糖体核糖核酸的化学发光核酸探针检测法已投放临床实验室使用（AccuProbe淋病奈瑟菌检测法）。在本研究中，将三种协同凝集试验（GonoGen I、Meritec GC和GC Omni）、GonoGen II免疫过滤法以及Micro Trak淋病奈瑟菌荧光单克隆抗体检测法与AccuProbe检测法进行比较，以鉴定淋球菌。所检测的菌株（n = 376）包括194株淋病奈瑟菌、82株脑膜炎奈瑟菌、32株乳糖奈瑟菌、32株其他奈瑟菌属、32株卡他莫拉菌、2株莫拉克斯氏菌属和2株反硝化金氏杆菌。GonoGen I、Meritec GC和GC Omni协同凝集试验分别对93.8%、92.3%和95.9%的淋球菌产生明显阳性结果。GonoGen II明确鉴定出91.8%的淋球菌，MicroTrak荧光抗体检测法鉴定出90.7%荧光强度为2+或更高的淋球菌。AccuProbe鉴定出所检测的100%的淋球菌。GonoGen I和GonoGen II的特异性为98%，Meritec GC的特异性为99%，GC Omni、MicroTrak荧光抗体检测法和AccuProbe检测法的特异性均为100%。虽然基于抗体的检测法在结果易于解释时是可靠的，但AccuProbe是唯一一种100%准确的确诊检测法。血清分型研究表明，一系列β-内酰胺酶阳性和阴性淋球菌血清型通常不与基于单克隆抗体的检测法反应，尤其是不与荧光抗体检测法反应。
