Janda W M, Sobieski V
Department of Medical Laboratory Sciences, University of Illinois, Chicago.
Eur J Clin Microbiol Infect Dis. 1988 Feb;7(1):25-9. doi: 10.1007/BF01962166.
A ten-minute chromogenic substrate test was evaluated for its ability to rapidly identify pathogenic Neisseria spp. and Branhamella catarrhalis. Identifications obtained with this system were compared to those obtained using conventional procedures. The test correctly identified 98.9% of 90 Neisseria gonorrhoeae, 98.3% of 60 Neisseria meningitidis, 96.2% of 26 Neisseria lactamica, and 100% of 36 Branhamella catarrhalis strains. Eight Neisseria subflava strains that grew on modified Thayer-Martin agar were prolyl aminopeptidase positive and were misidentified as Neisseria gonorrhoeae. Other strains of saprophytic Neisseria spp. also reacted with the chromogenic substrates. The system was accurate and reliable for identifying the commonly encountered pathogenic species. In light of recent reports describing new species and atypical Neisseria strains, however, careful attention to the salient features of both common and atypical organisms is necessary for proper use of rapid enzymatic identification tests.
对一项10分钟的显色底物试验进行了评估，以确定其快速鉴定致病性奈瑟菌属和卡他布兰汉菌的能力。将该系统获得的鉴定结果与使用传统方法获得的结果进行比较。该试验正确鉴定出90株淋病奈瑟菌中的98.9%、60株脑膜炎奈瑟菌中的98.3%、26株乳酸奈瑟菌中的96.2%以及36株卡他布兰汉菌中的100%。在改良的Thayer-Martin琼脂上生长的8株微黄奈瑟菌菌株脯氨酰氨肽酶呈阳性，并被误鉴定为淋病奈瑟菌。其他腐生性奈瑟菌属菌株也与显色底物发生反应。该系统在鉴定常见的致病菌种方面准确可靠。然而，鉴于最近有报道描述了新菌种和非典型奈瑟菌菌株，为正确使用快速酶促鉴定试验，必须仔细关注常见和非典型生物体的显著特征。
