Pitkänen A, Amaral D G
University of Kuopio, Department of Neurology, Finland.
J Comp Neurol. 1993 May 8;331(2):199-224. doi: 10.1002/cne.903310205.
Calbindin-D28k is a calcium-binding protein located in a variety of neuronal cell types in many regions of the central nervous system. In the present study, we describe the distribution of calbindin-D28k-immunoreactive cells, fibers, and terminals in the monkey amygdaloid complex. Calbindin-D28k-immunoreactive neurons could be divided into four major cell types. Neurons of the first three cell types demonstrated clearly stained dendrites that were either aspiny or had a few spines on their distal portions. Type 1 cells were small, stellate, or multipolar and found throughout the amygdala. Type 2 cells were large, multipolar and were most commonly found in the deep nuclei, particularly in the lateral nucleus, intermediate division of the basal nucleus, accessory basal nucleus and in the periamygdaloid cortex. Type 3 cells were fusiform, of various sizes, and were found throughout the amygdala. Type 4 cells were quite large and lightly stained; the dendrites of these cells were usually unstained. The size, shape, and location of Type 4 labeled cell bodies suggested that they might be the large, modified pyramidal cells that constitute the projection neurons of the amygdala. Type 4 cells were observed primarily in the lateral, basal, and accessory basal nuclei and in the periamygdaloid cortex. Calbindin-D28k-immunoreactive fibers and terminals were difficult to observe in the amygdala partly because of a diffuse, finely granular neuropil labeling that was particularly dense in the anterior cortical and medial nuclei, in the central nucleus, and in the periamygdaloid cortex. The neuropil labeling was substantially lighter in the lateral, basal, and accessory basal nuclei. Conspicuous linear profiles resembling the "calbindin bundles" of the neocortex were evident in large numbers in the accessory basal nucleus, the medial portion of the parvicellular division of the basal nucleus, in the amygdalohippocampal area, and in the periamygdaloid cortex. There were calbindin-D28k-positive fibers in the stria terminalis and in the ventral amygdalofugal pathway. When the distributions of calbindin-D28k and parvalbumin immunoreactivity in the monkey amygdaloid complex were compared, it appeared that the overall distribution of these two calcium-binding proteins was generally complementary rather than overlapping.
钙结合蛋白-D28k是一种钙结合蛋白，位于中枢神经系统许多区域的多种神经元细胞类型中。在本研究中，我们描述了钙结合蛋白-D28k免疫反应性细胞、纤维和终末在猴杏仁核复合体中的分布。钙结合蛋白-D28k免疫反应性神经元可分为四种主要细胞类型。前三种细胞类型的神经元显示出明显染色的树突，这些树突要么无棘，要么在其远端部分有一些棘。1型细胞小，呈星形或多极，见于整个杏仁核。2型细胞大，呈多极，最常见于深部核团，特别是外侧核、基底核中间部、副基底核和杏仁周皮质。3型细胞呈梭形，大小不一，见于整个杏仁核。4型细胞相当大，染色浅；这些细胞的树突通常未染色。4型标记细胞体的大小、形状和位置表明它们可能是构成杏仁核投射神经元的大的、改良的锥体细胞。4型细胞主要见于外侧核、基底核和副基底核以及杏仁周皮质。在杏仁核中很难观察到钙结合蛋白-D28k免疫反应性纤维和终末，部分原因是存在弥漫性、细颗粒状的神经毡标记，在前皮质核和内侧核、中央核以及杏仁周皮质中尤为密集。在外侧核、基底核和副基底核中，神经毡标记明显较轻。在副基底核、基底核小细胞部内侧、杏仁海马区和杏仁周皮质中大量可见类似于新皮质“钙结合蛋白束”的明显线性轮廓。终纹床核和腹侧杏仁体传出通路中有钙结合蛋白-D28k阳性纤维。当比较猴杏仁核复合体中钙结合蛋白-D28k和小白蛋白免疫反应性的分布时，这两种钙结合蛋白的总体分布似乎通常是互补的而非重叠的。
