Richmond M D, Yeung E S
Ames Laboratory-USDOE, Iowa 50011.
Anal Biochem. 1993 May 1;210(2):245-8. doi: 10.1006/abio.1993.1190.
The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displace the anionic fluorescing buffer ion to create negative peaks. Detection limits in the picogram range are possible.
介绍了在毛细管区带电泳中对天然多糖的分离和检测。为了提供电泳迁移率的差异，使用pH值为11.5来使分析物部分电离。通过间接荧光进行检测，带电分析物取代阴离子荧光缓冲离子以产生负峰，从而实现检测。皮克级的检测限是可能的。
