Sirichai S, de Mello A J
AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology and Medicine, South Kensington, London, UK.
Electrophoresis. 2001 Jan;22(2):348-54. doi: 10.1002/1522-2683(200101)22:2<348::AID-ELPS348>3.0.CO;2-4.
The separation and detection of both print and film developing agents (CD-3 and CD-4) in photographic processing solutions using chip-based capillary electrophoresis is presented. For simultaneous detection of both analytes under identical experimental conditions a buffer pH of 11.9 is used to partially ionise the analytes. Detection is made possible by indirect fluorescence, where the ions of the analytes displace the anionic fluorescing buffer ion to create negative peaks. Under optimal conditions, both analytes can be analyzed within 30 s. The limits of detection for CD-3 and CD-4 are 0.17 mM and 0.39 mM, respectively. The applicability of the method for the analysis of seasoned photographic processing developer solutions is also examined.
介绍了使用基于芯片的毛细管电泳法对摄影冲洗液中的冲印剂和显影剂（CD - 3和CD - 4）进行分离和检测。为了在相同实验条件下同时检测两种分析物，使用pH值为11.9的缓冲液使分析物部分离子化。通过间接荧光进行检测，分析物的离子取代阴离子荧光缓冲离子以产生负峰。在最佳条件下，两种分析物均可在30秒内完成分析。CD - 3和CD - 4的检测限分别为0.17 mM和0.39 mM。还考察了该方法在分析陈旧摄影冲洗显影液中的适用性。
