Mori H, Yamakura T, Masaki H, Mishina M
Department of Neuropharmacology, Niigata University, Japan.
Neuroreport. 1993 May;4(5):519-22. doi: 10.1097/00001756-199305000-00014.
The epsilon 1/zeta 1 and epsilon 2/zeta 1 heteromeric N-methyl-D-aspartate (NMDA) receptor channels expressed in Xenopus oocytes, but not the epsilon 3/zeta 1 and epsilon 4/zeta 1 channels, are positively modulated by the treatment with 12-O-tetradecanoyl phorbol 13-acetate (TPA). Failure of potentiation in the presence of staurosporine suggests the involvement of protein kinases in the TPA effect. To identify the structural domain involved in the modulation of the NMDA receptor channel by the TPA treatment, we constructed chimeric subunits between the epsilon 2 and epsilon 3 subunits. Functional analysis of heteromeric channels containing chimeric epsilon subunits has shown that the carboxyl-terminal region of the epsilon 2 subunit is responsible for the activation of the epsilon 2/zeta 1 channel by the TPA treatment.
在非洲爪蟾卵母细胞中表达的ε1/ζ1和ε2/ζ1异聚体N-甲基-D-天冬氨酸（NMDA）受体通道，而非ε3/ζ1和ε4/ζ1通道，在用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理后受到正向调节。在存在星形孢菌素的情况下增强作用失败，提示蛋白激酶参与了TPA效应。为了鉴定TPA处理对NMDA受体通道调节所涉及的结构域，我们构建了ε2和ε3亚基之间的嵌合亚基。对含有嵌合ε亚基的异聚体通道进行功能分析表明，ε2亚基的羧基末端区域负责TPA处理对ε2/ζ1通道的激活。
