Yamazaki M, Mori H, Araki K, Mori K J, Mishina M
Department of Neuropharmacology, Niigata University, Japan.
FEBS Lett. 1992 Mar 23;300(1):39-45. doi: 10.1016/0014-5793(92)80160-i.
The primary structure and presence of two forms of the mouse N-methyl-D-aspartate (NMDA) receptor channel subunit zeta 1 have been disclosed by cloning and sequencing the cDNAs. The zeta 1 subunit shows approximately 20% amino acid sequence identities with the rodent alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)- or kainate-selective GluR subunits and has structural features common to neurotransmitter-gated ion channels. Functional homomeric zeta 1 channels expressed in Xenopus oocytes by injection of the subunit-specific mRNA exhibit current responses characteristic for the NMDA receptor channel such as activation by glycine, Ca2+ permeability, blocking by Mg2+ and activation by polyamine. It has been found that the zeta 1 channel activity is positively modulated by treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA).
通过对cDNA进行克隆和测序，已揭示了小鼠N-甲基-D-天冬氨酸（NMDA）受体通道亚基zeta 1的两种形式的一级结构和存在情况。zeta 1亚基与啮齿动物α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）或红藻氨酸选择性GluR亚基显示出约20%的氨基酸序列同一性，并且具有神经递质门控离子通道共有的结构特征。通过注射亚基特异性mRNA在非洲爪蟾卵母细胞中表达的功能性同聚zeta 1通道表现出NMDA受体通道特有的电流反应，如被甘氨酸激活、Ca2+通透性、被Mg2+阻断以及被多胺激活。已发现zeta 1通道活性可通过用12-O-十四烷酰佛波醇13-乙酸酯（TPA）处理而得到正向调节。
