Suzuki Y, Takeda M
Department of Oral Anatomy, School of Dentistry, Higashi Nippon Gakuen University, Hokkaido, Japan.
Brain Res Dev Brain Res. 1993 May 21;73(1):107-13. doi: 10.1016/0165-3806(93)90052-c.
The basal region in the developing olfactory epithelium of mice was investigated by double immunostaining using anti-keratin (MA903) and anti-bromodeoxyuridine antibodies. Basal cells proper, which are MA903 positive, and globose basal cells, which are MA903 negative, were differentiated in the basal region on embryonic day 18 to postnatal day 1. At this stage, BrdU-labeling was found more in the glubose basal cells than in the basal cells proper. The labeled globose basal cells increased in number and reached the maximum on postnatal day 3, and decreased in number on postnatal day 14. The labeled basal cells proper was concentrated near the border with the respiratory epithelium on postnatal day 1, but they were found in small numbers in other regions of olfactory epithelium during later stages of development. Active division of globose basal cells accompanied the 1.5-fold increase in the thickness of the olfactory epithelium, which is caused by the increase in the number of olfactory cells, during development. It is suggested that during late embryonic and postnatal days olfactory cells originate from globose basal cells, not from basal cells proper. The division of basal cells proper may contribute to an 8-fold increase in the surface area of the olfactory epithelium during development.
利用抗角蛋白（MA903）和抗溴脱氧尿苷抗体进行双重免疫染色，对发育中小鼠嗅觉上皮的基底区域进行了研究。在胚胎第18天至出生后第1天的基底区域，可区分出MA903阳性的固有基底细胞和MA903阴性的球状基底细胞。在此阶段，发现溴脱氧尿苷（BrdU）标记在球状基底细胞中比在固有基底细胞中更多。标记的球状基底细胞数量增加，并在出生后第3天达到最大值，在出生后第14天数量减少。出生后第1天，标记的固有基底细胞集中在与呼吸上皮的边界附近，但在发育后期的嗅觉上皮其他区域数量较少。在发育过程中，球状基底细胞的活跃分裂伴随着嗅觉上皮厚度增加1.5倍，这是由嗅觉细胞数量增加引起的。提示在胚胎后期和出生后，嗅觉细胞起源于球状基底细胞，而非固有基底细胞。固有基底细胞的分裂可能有助于发育过程中嗅觉上皮表面积增加8倍。
