Suzuki Y, Takeda M, Obara N, Suzuki N
Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.
Anat Embryol (Berl). 1998 Jul;198(1):43-51. doi: 10.1007/s004290050163.
The cytotoxic agent colchicine induced apoptotic cell death and subsequent regeneration in the mouse olfactory epithelium and vomeronasal organ. The TUNEL method revealed the presence of many apoptotic bodies in the middle to basal region of the septal olfactory epithelium and vomeronasal organ near the boundary of the respiratory epithelium at 1 day after a single i.p. injection of colchicine (4 mg/kg b.w.). In some regions of the third and the fourth nasal turbinates, massive apoptosis was observed in the olfactory epithelium. Electron micrographs of the septum showed that immature olfactory cells and globose basal cells were killed by the colchicine and had been phagocytized by the supporting cells and macrophages. In the vomeronasal organ, immature sensory cells and precursors died in response to the colchicine. In response to cell death, active proliferation of precursor cells (globose basal cells) and subsequent regeneration of olfactory cells occurred in the olfactory epithelium and vomeronasal organ. Incorporation of the mitotic tracer BrdU by precursor cells reached its peak at 4 days after colchicine treatment in the vomeronasal organ, and at 6 to 7 days in the olfactory epithelium; however, in some regions in the third and the fourth nasal turbinates, where many olfactory cells and globose basal cells had died by colchicine effect, the regeneration did not occur even in 1 month, forming the epithelium of only supporting cells and horizontal basal cells. In the next month, these regions became normal olfactory epithelium. This suggests that the globose basal cells in the surrounding normal olfactory epithelium might invade these regions to give rise to the olfactory cells.
细胞毒性药物秋水仙碱可诱导小鼠嗅觉上皮和犁鼻器发生凋亡性细胞死亡及随后的再生。采用TUNEL法检测发现，单次腹腔注射秋水仙碱（4mg/kg体重）1天后，在鼻中隔嗅觉上皮和犁鼻器靠近呼吸上皮边界的中基区域存在许多凋亡小体。在第三和第四鼻甲的一些区域，嗅觉上皮中观察到大量凋亡。鼻中隔的电子显微镜图像显示，未成熟的嗅觉细胞和球状基底细胞被秋水仙碱杀死，并被支持细胞和巨噬细胞吞噬。在犁鼻器中，未成熟的感觉细胞和前体细胞因秋水仙碱作用而死亡。作为对细胞死亡的反应，前体细胞（球状基底细胞）在嗅觉上皮和犁鼻器中活跃增殖，随后嗅觉细胞再生。秋水仙碱处理后4天，犁鼻器中前体细胞对有丝分裂示踪剂BrdU的掺入达到峰值，而在嗅觉上皮中则在6至7天达到峰值；然而，在第三和第四鼻甲的一些区域，许多嗅觉细胞和球状基底细胞因秋水仙碱作用而死亡，即使在1个月后也未发生再生，仅形成由支持细胞和水平基底细胞组成的上皮。在下一个月，这些区域恢复为正常的嗅觉上皮。这表明周围正常嗅觉上皮中的球状基底细胞可能侵入这些区域并产生嗅觉细胞。
