Kontsek P, Borecký L, Zav'yalov V P, Maiorov V A
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Immunol Lett. 1993 Mar;35(3):281-4. doi: 10.1016/0165-2478(93)90195-8.
Immunologically less reactive but functionally relevant structures were identified on human interferon (IFN)-alpha 2 by three neutralizing monoclonal antibodies (mAb). The binding sites of these mAbs were mapped using a set of synthetic peptides that covered the amino acid sequence of two predicted biologically active segments in the regions 31-53 and 63-85 of IFN-alpha 2. We measured the capacity of fragments to inhibit the IFN-neutralizing activity of mAbs and located three linear epitopes around residues 42-53, 63-76 and 77-85 of the IFN-alpha 2 molecule.
