Conrads G, Brauner A
Department of Conservation, Periodontology and Preventive Dentistry, Technical University (RWTH), Aachen, FRG.
FEMS Immunol Med Microbiol. 1993 Mar;6(2-3):115-20. doi: 10.1111/j.1574-695X.1993.tb00313.x.
Digoxigenin-labelled synthetic DNA probes directed against the 16S rRNA were used for the direct detection of the periodontopathogenic bacteria Prevotella intermedia and Porphyromonas gingivalis in subgingival plaque by applying a DNA-RNA dot-blot hybridization procedure. The test was evaluated with 134 plaque samples from 26 patients with adult periodontitis or rapidly progressive periodontitis. The lower limit of detection was 10(4)-10(5) bacteria/specimen. A semiquantitative assessment of the two species in each sample and in the corresponding periodontal site was achieved by this technique. It is possible to examine 80-90 samples within two days with low material costs.
采用DNA - RNA斑点杂交技术，用针对16S rRNA的地高辛标记合成DNA探针，直接检测龈下菌斑中的牙周病原菌中间普氏菌和牙龈卟啉单胞菌。对26例成人牙周炎或快速进展性牙周炎患者的134份菌斑样本进行了该检测。检测下限为10(4)-10(5)个细菌/样本。通过该技术可对每个样本及其相应牙周部位的这两种菌进行半定量评估。该技术能够在两天内检测80 - 90个样本，且材料成本较低。
