荧光原位杂交技术用于直接观察龈下菌斑样本中的革兰氏阴性厌氧菌。
Fluorescence in situ hybridization for direct visualization of gram-negative anaerobes in subgingival plaque samples.
作者信息
Gersdorf H, Pelz K, Göbel U B
机构信息
Institut für Medizinische Mikrobiologie und Hygiene, Klinikum der Albert-Ludwigs-Universität, Freiburg, FRG.
出版信息
FEMS Immunol Med Microbiol. 1993 Mar;6(2-3):109-14. doi: 10.1111/j.1574-695X.1993.tb00312.x.
Abstract
Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.
摘要
使用与牙龈卟啉单胞菌和福赛斯坦纳菌16S核糖体RNA可变区互补的荧光寡核苷酸探针，在患有牙周炎患者的甲醛固定龈下菌斑样本涂片上鉴定这些微生物。荧光原位杂交是评估牙周菌群微生物生态学的一种有用方法。
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