Mohankumar P S, Thyagarajan S, Quadri S K
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
Brain Res Bull. 1993;31(6):745-8. doi: 10.1016/0361-9230(93)90151-z.
Push-pull perfusion technique was used to infuse interleukin-1 beta (IL-1 beta) into and collect perfusate from the medial basal hypothalamus (MBH) of conscious, freely moving rats. The serotonin metabolite, 5-hydroxyindoleacetic acid (5-HIAA), was measured in the perfusate by high performance liquid chromatography. Infusion of the vehicle, PBS-0.1% BSA, had no significant effect on 5-HIAA release except near the end of the perfusion period (325 min) when the release was below the pretreatment level (p < 0.05). Infusion of 25 ng of IL-1 beta prevented this decrease, whereas infusion of 50 ng produced an increase of more than 50% (p < 0.05) at 25 min and maintained it at that level during the remaining posttreatment period. In the animals infused with 100 ng of IL-1 beta, 5-HIAA release increased by more than 70% at 25 min and was more than 120% (p < 0.05) above the pretreatment level at the end of the posttreatment period. We concluded that IL-1 beta affects the metabolism of serotonergic system in the hypothalamus and that this is a component of the mechanism by which IL-1 produces its central actions.
采用推挽灌注技术，将白细胞介素-1β（IL-1β）注入清醒、自由活动大鼠的内侧基底部下丘脑（MBH）并从中收集灌流液。通过高效液相色谱法测定灌流液中5-羟色胺代谢产物5-羟吲哚乙酸（5-HIAA）的含量。注入溶媒PBS-0.1%牛血清白蛋白（BSA）对5-HIAA的释放没有显著影响，除非在灌注期接近结束时（325分钟），此时释放量低于预处理水平（p<0.05）。注入25 ng的IL-1β可防止这种下降，而注入50 ng在25分钟时可使释放量增加超过50%（p<0.05），并在剩余的治疗后时期维持在该水平。在注入100 ng IL-1β的动物中，5-HIAA的释放在25分钟时增加超过70%，在治疗后时期结束时比预处理水平高出超过120%（p<0.05）。我们得出结论，IL-1β影响下丘脑5-羟色胺能系统的代谢，这是IL-1产生其中枢作用机制的一个组成部分。
