Mohankumar P S, Thyagarajan S, Quadri S K
Department of Anatomy & Physiology, Kansas State University, Manhattan 66506.
Life Sci. 1991;48(9):925-30. doi: 10.1016/0024-3205(91)90040-i.
Push-pull perfusion technique was used to infuse IL-1 beta into and collect perfusate from the medial basal hypothalamus of freely moving male rats. Dopamine (DA) and its metabolite, dihydroxyphenylacetic acid (DOPAC), were measured in the perfusate using high performance liquid chromatography with electrochemical detection. In the control group, release rates of DA and DOPAC decreased and were 62% and 40%, respectively, below pretreatment levels after 325 min. In contrast, treatment with 50 ng of IL-1 beta produced substantial reductions in these decreases, and treatment with 100 ng of IL-1 beta produced increases of up to 118% and 89% in the release rates of DA and DOPAC, respectively. It is concluded that IL-1 beta affects the metabolism of catecholamines (and probably other neurotransmitters) in the brain, which, in turn, mediate its central and neuroendocrine actions.
采用推挽灌注技术，将白细胞介素-1β注入自由活动的雄性大鼠内侧基底下丘脑并收集灌流液。使用高效液相色谱-电化学检测法测定灌流液中的多巴胺（DA）及其代谢产物二羟基苯乙酸（DOPAC）。对照组中，325分钟后，DA和DOPAC的释放率下降，分别比预处理水平低62%和40%。相比之下，用50纳克白细胞介素-1β处理可显著减少这些下降，用100纳克白细胞介素-1β处理则使DA和DOPAC的释放率分别提高了118%和89%。结论是，白细胞介素-1β影响大脑中儿茶酚胺（可能还有其他神经递质）的代谢，进而介导其中枢和神经内分泌作用。
