Botney M D, Liptay M J, Kaiser L R, Cooper J D, Parks W C, Mecham R P
Department of Medicine, Jewish Hospital, Washington University Medical Center, St. Louis, MO 63110.
Am J Pathol. 1993 Jul;143(1):121-9.
Immunohistochemistry was performed on lung tissue obtained from patients with severe unexplained pulmonary hypertension using an antibody to the amino-terminal end of the procollagen type I propeptide. This antibody identifies newly synthesized alpha I(I) procollagen before cleavage of the amino-terminal propeptide following secretion and, therefore, can identify sites of active collagen deposition. Procollagen was detected in the media, media and neointima, or neointima alone of a large number of small muscular arteries from hypertensive lungs. Normal adult lungs were negative. Neointimal cells in remodeled small muscular arteries stained positively for alpha-smooth muscle actin and desmin consistent with a smooth muscle lineage. These data suggest smooth muscle-like cells in small muscular arteries are actively synthesizing collagen in patients with severe unexplained pulmonary hypertension.
使用针对I型前胶原前肽氨基末端的抗体，对患有严重不明原因肺动脉高压的患者所获取的肺组织进行免疫组织化学检测。该抗体可识别分泌后氨基末端前肽裂解之前新合成的αI（I）前胶原，因此能够识别活性胶原沉积部位。在高血压肺的大量小肌性动脉的中膜、中膜和新内膜或仅新内膜中检测到前胶原。正常成人肺组织呈阴性。重塑的小肌性动脉中的新内膜细胞α-平滑肌肌动蛋白和结蛋白染色呈阳性，符合平滑肌谱系特征。这些数据表明，在患有严重不明原因肺动脉高压的患者中，小肌性动脉中的平滑肌样细胞正在积极合成胶原。
