银环蛇毒素可检测AMPA受体通道中的分子差异。
Argiotoxin detects molecular differences in AMPA receptor channels.
作者信息
Herlitze S, Raditsch M, Ruppersberg J P, Jahn W, Monyer H, Schoepfer R, Witzemann V
机构信息
Abteilung Zellphysiologie, Max-Planck-Institut für medizinische Forschung, Heidelberg, Federal Republic of Germany.
出版信息
Neuron. 1993 Jun;10(6):1131-40. doi: 10.1016/0896-6273(93)90061-u.
Argiotoxin, a component of the spider venom from Argiope lobata, blocks AMPA receptor channels expressed in homomeric and heteromeric configuration in Xenopus oocytes. Argiotoxin acts as an open channel blocker in a voltage-dependent manner and discriminates between the functionally diverse AMPA receptors. Importantly, a transmembrane region 2 determinant for divalent cation permeability also determines argiotoxin sensitivity. Subunit-specific differences in the time courses of block and recovery demonstrate that heteromeric AMPA receptors can assemble in variable ratios. Thus, argiotoxin can be used as a tool in analyzing the subunit composition of AMPA receptors in native membranes.
精毒素是大腹园蛛毒液的一种成分，它能阻断非洲爪蟾卵母细胞中以同聚体和异聚体形式表达的AMPA受体通道。精毒素以电压依赖的方式作为一种开放通道阻滞剂，并且能区分功能多样的AMPA受体。重要的是，决定二价阳离子通透性的跨膜区2也决定了精毒素敏感性。阻断和恢复时间过程中的亚基特异性差异表明，异聚体AMPA受体可以以不同比例组装。因此，精毒素可作为一种工具用于分析天然膜中AMPA受体的亚基组成。
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