Pokholok D K, Gudima S O, Yesipov D S, Dobrynin V N, Rechinsky V O, Kochetkov S N
V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
FEBS Lett. 1993 Jul 5;325(3):237-41. doi: 10.1016/0014-5793(93)81080-j.
To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated with the reduced capability of AZT-TP to block the DNA- but not RNA-directed DNA synthesis.
