Ekström T J, Bäcklin B M, Lindqvist Y, Engström W
Centre for Biotechnology, NOVUM Research Park, Huddinge, Sweden.
Gen Comp Endocrinol. 1993 May;90(2):243-50. doi: 10.1006/gcen.1993.1079.
Mink cDNA for insulin-like growth factor II (IGF-II) has been isolated by cDNA synthesis from bulk mRNA and subsequent PCR-screening. Positive clones were sequenced and analyzed. Analysis of a cDNA revealed that, compared with human, the mature mink IGF-II peptide contains 68 instead of 67 amino acids with a serine insertion at residue 40. With this exception, the homology between the human and mink mature peptide is 100% and is 94% between rat and mink. Comparing IGF-II transcripts in fetal and adult mink liver, fetal tissue contains three transcripts of 5.8, 4.5, and 4.0 kb. In contrast, adult liver expresses low levels of a 4.6-kb transcript.
通过从大量mRNA进行cDNA合成及随后的PCR筛选，分离出了水貂胰岛素样生长因子II（IGF-II）的cDNA。对阳性克隆进行了测序和分析。一个cDNA的分析表明，与人类相比，成熟水貂IGF-II肽含有68个而非67个氨基酸，在第40位残基处有一个丝氨酸插入。除此之外，人类和水貂成熟肽之间的同源性为100%，大鼠和水貂之间为94%。比较胎儿和成年水貂肝脏中的IGF-II转录本，胎儿组织含有5.8、4.5和4.0 kb的三种转录本。相比之下，成年肝脏表达低水平的4.6-kb转录本。
