Liu X H, Yoshiki T, Kokuho M, Okada Y, Tomoyoshi T, Higuchi K
Department of Urology, Shiga University of Medical Science.
Hinyokika Kiyo. 1993 May;39(5):439-44.
The anti-HNK-1 (Leu-7) monoclonal antibody (MAb) was revealed to be reactive with noncancerous and cancerous prostatic epithelial cells, although this antibody was originally found to be reactive against natural killer cells. However, the prognostic significance of HNK-1 antigen in prostatic cancer patients remains unknown. The expression of HNK-1 antigen on prostatic cancer was investigated immunohistochemically using the avidin-biotin-peroxidase complex (ABC) method with the anti-HNK-1 monoclonal antibody. Of the 52 patients with prostatic cancer, 49 patients (94%) showed reactivity to anti-HNK-1 MAb and the immunoreaction was associated with the histological differentiation of prostatic cancer. Well differentiated cancer showed the highest percentage of positively stained cancer cells and poorly differentiated cancer showed the lowest percentage. No statistically significant differences existed between groups classified by stage, although the more advanced cancers tended to have weaker reactions. The five-year survival rate and interval free of progression were then studied using the Kaplan-Meier method on 33 patients with stage D2 disease who had received endocrine therapy. The findings indicated that a high survival rate and a longer interval free of progression were associated with a higher fraction of positively stained cancer cells. In conclusion, the expression of HNK-1 antigen on prostatic cancer may be a useful prognostic factor in patients with prostatic cancer.
抗HNK-1（Leu-7）单克隆抗体（MAb）被发现可与非癌性和癌性前列腺上皮细胞发生反应，尽管该抗体最初被发现可与自然杀伤细胞发生反应。然而，HNK-1抗原在前列腺癌患者中的预后意义仍然未知。使用抗HNK-1单克隆抗体，采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，通过免疫组织化学研究了前列腺癌上HNK-1抗原的表达。在52例前列腺癌患者中，49例（94%）显示对抗HNK-1 MAb有反应，且免疫反应与前列腺癌的组织学分化相关。高分化癌显示阳性染色癌细胞的百分比最高，低分化癌显示的百分比最低。按分期分类的组间无统计学显著差异，尽管进展期癌症往往反应较弱。然后，使用Kaplan-Meier方法对33例接受内分泌治疗的D2期疾病患者进行了五年生存率和无进展间期的研究。结果表明，高生存率和较长的无进展间期与较高比例的阳性染色癌细胞相关。总之，前列腺癌上HNK-1抗原的表达可能是前列腺癌患者有用的预后因素。
