Radermecker M F, Leclercq M D, Mariz S D, Louis R E
Department of Respiratory Medicine, University of Liège, Belgium.
Int Arch Allergy Immunol. 1993;101(3):283-7. doi: 10.1159/000236459.
Basophils in about 15% of subjects allergic to hymenoptera venom do not release histamine in the presence of antigen. Little is known on the basophil releasability in these patients. We therefore measured maximum percent leukocyte histamine release to antigen (Vespula venom), anti-IgE and formylmethionylphenylalanine (FMP) in 39 patients allergic to wasp venom and compared our results according to basophil responsiveness to antigen. Mean maximum percent histamine release was 39, 34 and 22%, respectively, for venom (100 ng/ml), anti-IgE (0.25 microgram/ml) and FMP (10(-4) M). The amount of histamine specifically released by venom correlated significantly with anti-IgE but not with FMP-induced histamine release. Leukocytes were unresponsive to antigen in 10 subjects. The clinical characteristics and anaphylactic symptoms of these patients were not different from those with antigen-responsive cells. Unresponsive leukocytes responded to FMP in all and to anti-IgE in 8 of the 10 subjects. Mean anti-IgE and FMP-induced histamine release were, respectively, lower and higher than those observed with leukocytes responsive to antigen (p < 0.05). In unresponsive basophils, there was a negative correlation between maximum percent anti-IgE and FMP-induced histamine release. We confirm that basophils of a minority of the subjects allergic to Vespula venom do not release histamine in the presence of antigen. The negative correlation between anti-IgE and FMP-induced histamine release in unresponsive basophils may suggest individual differences in the ratio of Fc epsilon RI and FMP receptors on the surface of basophils.
在约15%对膜翅目毒液过敏的受试者中，嗜碱性粒细胞在抗原存在时不释放组胺。对于这些患者的嗜碱性粒细胞释放能力了解甚少。因此，我们测定了39例对黄蜂毒液过敏患者对抗原（黄蜂毒液）、抗IgE和甲酰甲硫氨酰苯丙氨酸（FMP）的最大白细胞组胺释放百分比，并根据嗜碱性粒细胞对抗原的反应性比较了我们的结果。对于毒液（100 ng/ml）、抗IgE（0.25微克/ml）和FMP（10⁻⁴ M），平均最大组胺释放百分比分别为39%、34%和22%。毒液特异性释放的组胺量与抗IgE显著相关，但与FMP诱导的组胺释放无关。10名受试者的白细胞对抗原无反应。这些患者的临床特征和过敏症状与抗原反应性细胞的患者无异。10名受试者中，无反应的白细胞对FMP均有反应，8名对抗IgE有反应。抗IgE和FMP诱导的平均组胺释放分别低于和高于对抗原有反应的白细胞所观察到的水平（p < 0.05）。在无反应的嗜碱性粒细胞中，抗IgE和FMP诱导的最大组胺释放百分比之间呈负相关。我们证实，少数对黄蜂毒液过敏的受试者的嗜碱性粒细胞在抗原存在时不释放组胺。无反应的嗜碱性粒细胞中抗IgE和FMP诱导的组胺释放之间的负相关可能提示嗜碱性粒细胞表面FcεRI和FMP受体比例的个体差异。
