Fujie K, Aoki T, Ito Y, Maeda S
Department of Natural Science, Osaka Women's University, Japan.
Mutat Res. 1993 Aug;300(3-4):241-6. doi: 10.1016/0165-1218(93)90056-j.
An in vitro sister-chromatid exchange (SCE) assay using rat erythroblastic leukemia cells was conducted with four major trihalomethanes (THMs): chloroform, CHCl3; dichlorobromomethane, CHCl2Br, dibromochloromethane, CHClBr2; bromoform, CHBr3. In the absence of S9 mix, CHBr3, CHClBr2 and CHCl2Br significantly induced SCEs in a clear dose-dependent manner, while CHCl3 did not significantly induce SCEs. On the other hand, the incidence of CHCl3-induced SCEs significantly increased, although the incidence of CHBr3-induced SCEs decreased by the addition of S9 mix. However, there was no difference between the incidence of SCEs induced by CHBr3, CHClBr2 or CHCl2Br in the absence of S9 mix and that in the presence of S9 mix. The addition of crude catechin to the SCE assay system suppressed the ability of CHCl3 or CHBr3 to induce SCEs but had no suppressive effect on the other THM-induced SCEs. The suppression of SCEs induced by CHCl3 or CHBr3 depended on the crude catechin dose.
使用大鼠红白血病细胞进行了一项体外姐妹染色单体交换（SCE）试验，试验对象为四种主要的三卤甲烷（THMs）：氯仿（CHCl3）、二氯溴甲烷（CHCl2Br）、二溴氯甲烷（CHClBr2）、溴仿（CHBr3）。在不存在S9混合物的情况下，CHBr3、CHClBr2和CHCl2Br能以明显的剂量依赖性方式显著诱导SCE，而CHCl3则不会显著诱导SCE。另一方面，添加S9混合物后，CHCl3诱导的SCE发生率显著增加，而CHBr3诱导的SCE发生率下降。然而，在不存在S9混合物和存在S9混合物的情况下，CHBr3、CHClBr2或CHCl2Br诱导的SCE发生率没有差异。向SCE试验系统中添加粗儿茶素可抑制CHCl3或CHBr3诱导SCE的能力，但对其他THM诱导的SCE没有抑制作用。CHCl3或CHBr3诱导的SCE的抑制作用取决于粗儿茶素的剂量。
