Ribeiro L R, Silva A R, Bautista A R, Costa S L, Sales L A, Rios A C, Salvadori D M
Laboratório de Toxicologia e Genética Toxicológica, Universidade Federal da Bahia, Salvador, BA, Brazil.
Mutat Res. 1993 Aug;300(3-4):253-8. doi: 10.1016/0165-1218(93)90058-l.
This work has evaluated the clastogenicity of six extracts (tea and aqueous extract of leaves, tea, aqueous and methanolic extracts of dried fruit, and tea of unripe fruit) obtained from Crotalaria retusa L. and three extracts (tea and methanolic extract of dried fruit, and tea of unripe fruit) obtained from Crotalaria mucronata Desv. The extracts were injected intraperitoneally into mice, and the animals were killed 24 h after treatment for preparation of bone marrow cells. The extracts obtained from fruits of Crotalaria retusa were found to cause a dose-dependent increase in the frequency of chromosomal aberrations in mice. On the other hand, no statistically significant increase in the frequency of aberrant cells was observed for the animals treated with leaf extracts obtained from Crotalaria retusa and with extracts from fruits of Crotalaria mucronata. The possibility that the pyrrolizidine alkaloid, monocrotaline, present in Crotalaria retusa exerts a clastogenic effect on mouse bone marrow cells is discussed. Our conclusion is based on studies using intraperitoneal treatments. Effects of oral exposure to extracts of Crotalaria retusa are unknown.
本研究评估了从猪屎豆（Crotalaria retusa L.）中获得的六种提取物（叶的茶提取物和水提取物、干果的茶提取物、水提取物和甲醇提取物以及未成熟果实的茶提取物）以及从尖叶猪屎豆（Crotalaria mucronata Desv.）中获得的三种提取物（干果的茶提取物和甲醇提取物以及未成熟果实的茶提取物）的致断裂性。将提取物腹腔注射到小鼠体内，处理24小时后处死动物以制备骨髓细胞。发现从猪屎豆果实中获得的提取物会导致小鼠染色体畸变频率呈剂量依赖性增加。另一方面，用从猪屎豆叶中获得的提取物以及用从尖叶猪屎豆果实中获得的提取物处理的动物，未观察到异常细胞频率有统计学上的显著增加。讨论了猪屎豆中存在的吡咯里西啶生物碱——野百合碱对小鼠骨髓细胞产生致断裂作用的可能性。我们的结论基于腹腔注射处理的研究。猪屎豆提取物经口服暴露后的影响尚不清楚。
