Hamasaki T, Sato T, Nagase H, Kito H
Department of Public Health, Gifu Pharmaceutical University, Japan.
Mutat Res. 1993 Aug;300(3-4):265-71. doi: 10.1016/0165-1218(93)90060-q.
The mutagenicity of 14 organotin compounds which have been reported to be environmental pollutants, their environmental metabolites and inorganic tin (SnCl4) was studied. The experiments were carried out by a modification of the conventional Salmonella assay. Each tested chemical was removed by washing the tested strain with phosphate buffer, before the strain with top agar was poured onto minimal glucose agar. By this method, we were able to estimate the mutagenicity of organotin compounds which had antibacterial activity. It was apparent that mono-n-butyltin oxide, n-butyltin trichloride, di-n-butyltin dichloride, tri-n-butyltin chloride, bis-(tri-n-butyltin)-oxide and dimethyltin dichloride were mutagens on Salmonella typhimurium TA100 and bis-(tri-n-butyltin)-oxide showed the highest mutagenicity. With S. typhimurium TA98, di-n-butyltin dichloride was found to be a mutagen.
对14种已被报道为环境污染物的有机锡化合物、它们的环境代谢产物以及无机锡（SnCl4）的致突变性进行了研究。实验是通过对传统沙门氏菌试验进行改进来进行的。在将含有顶层琼脂的菌株倾注到最低葡萄糖琼脂上之前，先用磷酸盐缓冲液洗涤受试菌株，以去除每种受试化学物质。通过这种方法，我们能够评估具有抗菌活性的有机锡化合物的致突变性。很明显，单正丁基氧化锡、正丁基三氯化锡、二正丁基二氯化锡、三正丁基氯化锡、双（三正丁基锡）氧化物和二甲基二氯化锡对鼠伤寒沙门氏菌TA100具有致突变性，且双（三正丁基锡）氧化物显示出最高的致突变性。对于鼠伤寒沙门氏菌TA98，发现二正丁基二氯化锡是一种致突变剂。
