Rodilla V
Nuclear Energy Board, Clonskeagh Square, Dublin, Republic of Ireland.
Mutat Res. 1993 Aug;300(3-4):281-91. doi: 10.1016/0165-1218(93)90062-i.
It has recently been described that cisplatin is an agent able to induce binucleated cells (BC) in cultured CHO cells. Both the origin and the significance of those cells within a population are unknown although several hypothesis have been suggested such as blocking of cytokinesis or cell fusion. Using interval photography we have found that at least two mechanisms are involved in the production of BC. These cells can arise in a culture as a result of an incomplete process of cell division, i.e. karyokinesis with incomplete cytokinesis or as a result of the mitotic division of a pre-existent BC. The mitotic division of a BC can give rise to different types of daughter cells. These BC sometimes enter mitosis but fail to divide and as a consequence they remain BC. When the process of division is successful (in the vast majority of cases), the results that have been found are either two mononucleated cells or one mononucleated and one binucleated cell. The possible implications and significance of BC and BC with micronuclei in a given population are discussed.
最近有研究表明，顺铂是一种能够在培养的CHO细胞中诱导产生双核细胞（BC）的药物。尽管已经提出了几种假说，如胞质分裂受阻或细胞融合，但这些细胞在群体中的起源和意义尚不清楚。通过间隔摄影，我们发现至少有两种机制参与了双核细胞的产生。这些细胞可以在培养物中由于细胞分裂过程不完全而产生，即核分裂而胞质分裂不完全，或者是由于预先存在的双核细胞的有丝分裂。双核细胞的有丝分裂可以产生不同类型的子细胞。这些双核细胞有时进入有丝分裂但未能分裂，因此它们仍然是双核细胞。当分裂过程成功时（在绝大多数情况下），发现的结果要么是两个单核细胞，要么是一个单核细胞和一个双核细胞。本文讨论了给定群体中双核细胞和带有微核的双核细胞的可能影响和意义。
