Rodilla V, Pellicer J A, Serrano A, Pertusa J
Nuclear Energy Board, Clonskeagh Square, Dublin, Republic of Ireland.
Mutat Res. 1993 Feb;291(1):35-41. doi: 10.1016/0165-1161(93)90015-r.
Chinese hamster ovary (CHO) cells were treated with a single dose (10 micrograms/ml) of cis-diamminodichloroplatinum (II) (cisplatin) for 1 h and the effect of the drug on the kinetics of proliferation of the cultures was studied. It was found that the drug produces a delay in the proliferation rates of the treated cultures. The induction of micronuclei and binucleated cells (BC) at different times after treatment have also been studied, and the ability of these cells to undergo DNA synthesis (measured as the ability to incorporate [3H]thymidine) is shown. It was also found that cisplatin induced a particular type of BC that contains one or more micronuclei rather than a pure population of BC. The results obtained show a possible relationship between micronuclei and BC. The possibility that some of the micronucleated cells evolve in subsequent cell divisions to BC with micronuclei is suggested.
用单剂量（10微克/毫升）的顺二氨二氯铂（II）（顺铂）处理中国仓鼠卵巢（CHO）细胞1小时，并研究该药物对培养物增殖动力学的影响。发现该药物会使处理过的培养物的增殖速率延迟。还研究了处理后不同时间微核和双核细胞（BC）的诱导情况，并展示了这些细胞进行DNA合成的能力（通过掺入[3H]胸苷的能力来衡量）。还发现顺铂诱导出一种特殊类型的BC，其含有一个或多个微核，而不是纯的BC群体。所获得的结果显示了微核与BC之间可能存在的关系。有人提出，一些含微核的细胞可能在随后的细胞分裂中演变成带有微核的BC。
