Galanin-immunoreactivity in the nucleus basalis of Meynert in the rat: age-related changes and differential response to lesion-induced cholinergic cell loss.

The neuropeptide Galanin (Gal) is known to play a functional role in the basal forebrain cholinergic system. In our study, the morphology and density of the Gal-immunoreactive (Gal-IR) fiber network within the cholinergic nucleus basalis of Meynert (NBM) was investigated 1, 3 and 6 months after stereotaxic lesion with quisqualic acid in young adult (3 months old) and late middle-aged (20 months old) rats. Quantitative densitometry showed a significantly reduced Gal-IR fiber network in 20-month-old control rats. After lesion-induced cholinergic cell loss, no further changes in Gal-IR were noted in this group of aging rats during the period of investigation. In contrast, young adult animals displayed a significant increase of Gal-IR fiber density 6 months after NBM lesion. However, no hyperinnervation of individual surviving cholinergic neurons was seen. The results obtained in an animal model of cholinergic deficit support the hypothesis of age-related neuroplasticity of specific transmitter and peptide systems. Adaptive changes in Gal may play a role for the modulation of cholinergic function and could be of importance in human age-related neurodegenerative disorders, i.e. Alzheimer's disease.