Suzuki M, Tanaka M, Iwase T, Naito Y, Sugimura H, Kino I
First Department of Pathology, Hamamatsu University School of Medicine, Japan.
Biochem Biophys Res Commun. 1993 Jul 15;194(1):187-93. doi: 10.1006/bbrc.1993.1802.
A human ovarian yolk sac tumor cDNA library was screened for homeobox genes with an oligonucleotide probe under low stringent condition. Three homeobox genes were isolated, two of which were identified as HHO.c1 and HB24. The third was highly homologous with the mouse Hox-8 gene and was designated as HOX-8. Studies on RNAs from 25 human tumor tissues and cell lines showed that the profile of HOX-8 expression was different from those of HHO.c1 and HB24. The expression of HOX-8 was not detected in hematopoietic tumor cells, in which HHO.c1 and HB24 were highly expressed. HOX-8 was expressed at higher levels in a variety of tumors of epithelial origin than in their corresponding normal tissues more frequently than HHO.c1 and HB24. All three homeobox genes were highly expressed in a yolk sac tumor, an immature tumor of gonadal origin. These results suggest that HOX-8 plays a more important role in human tumors of epithelial origin than those of hematopoietic origin.
利用寡核苷酸探针在低严格条件下筛选人卵巢卵黄囊瘤cDNA文库中的同源框基因。分离出三个同源框基因，其中两个被鉴定为HHO.c1和HB24。第三个与小鼠Hox - 8基因高度同源，被命名为HOX - 8。对来自25个人类肿瘤组织和细胞系的RNA研究表明，HOX - 8的表达谱与HHO.c1和HB24不同。在造血肿瘤细胞中未检测到HOX - 8的表达，而HHO.c1和HB24在造血肿瘤细胞中高表达。与HHO.c1和HB24相比，HOX - 8在多种上皮来源的肿瘤中比在其相应的正常组织中更频繁地高表达。这三个同源框基因在卵黄囊瘤（一种性腺来源的未成熟肿瘤）中均高表达。这些结果表明，HOX - 8在人类上皮来源的肿瘤中比造血来源的肿瘤中发挥更重要的作用。
