Sandhu G S, Kline B C, Bolander M E, Sarkar G
Department of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905.
Biotechniques. 1993 Jun;14(6):948-52.
This paper reports a novel method for the identification of nucleic acid target sequences when these targets have high sequence identity. Homologous genes are currently identified by sequencing. We hypothesize that by primer extension in the presence of selected nucleotides, genes with similar sequence can be identified by the length of the extension products on gel electrophoresis. This simple procedure eliminates the much-demanding process of sequencing. We term this process Arrested Primer Extension (APE). As a demonstration of the feasibility of this method, we have used APE to speciate a known set of cultured mycobacteria. There should be many other applications of this method.
本文报道了一种在核酸靶序列具有高度序列同一性时对其进行鉴定的新方法。目前通过测序来鉴定同源基因。我们推测，在存在选定核苷酸的情况下通过引物延伸，具有相似序列的基因可通过凝胶电泳上延伸产物的长度来鉴定。这个简单的程序省去了要求苛刻的测序过程。我们将这个过程称为终止引物延伸（APE）。作为该方法可行性的一个例证，我们已使用APE对一组已知的培养分枝杆菌进行分类鉴定。该方法应该还有许多其他应用。
