对源自麻风瘤、可培养且侵袭神经的分枝杆菌HI-75的16S核糖体RNA基因进行全DNA序列分析。

Complete DNA sequence analysis for 16S ribosomal RNA gene of the leproma-derived, cultivable and nerve-invading mycobacterium HI-75.

作者信息

Sakai T, Matsuo E, Wakizaka A

机构信息

Department of Biochemistry and Molecular Biology, Kyorin University School of Medicine, Tokyo, Japan.

出版信息

Int J Lepr Other Mycobact Dis. 1999 Mar;67(1):52-9.

PMID:10407629

Abstract

The complete 1493 nucleotide sequence of the 16SrRNA gene of the leproma-derived and cultivable mycobacterium HI-75 strain was analyzed to elucidate the taxonomic characteristics by direct sequencing of the polymerase chain reaction (PCR) products. The results revealed that the sequence of mycobacterium HI-75 was mostly similar to that of Mycobacterium scrofulaceum with 5 bases differences in the sequenced 1493 bases (0.35%) of the 16SrRNA gene. M. leprae differed from the strain with 47 bases (3.3%). Sasaki and Hamit reported the nerve-invasive activity of the inoculated mycobacterium HI-75 in nude mice or the 131I-treated immunocompromised Swiss mice. The results indicate that mycobacterium HI-75 could be a mutant of M. scrofulaceum possessing the ability to invade the peripheral nerve in addition to developing leproma-like lesions.

摘要

对麻风瘤来源且可培养的分枝杆菌HI - 75菌株的16S rRNA基因的1493个核苷酸完整序列进行了分析，通过对聚合酶链反应（PCR）产物进行直接测序来阐明其分类学特征。结果显示，分枝杆菌HI - 75的序列与瘰疬分枝杆菌的序列最为相似，在16S rRNA基因测序的1493个碱基中存在5个碱基差异（0.35%）。麻风分枝杆菌与该菌株有47个碱基（3.3%）的差异。佐佐木和哈米特报道了接种的分枝杆菌HI - 75在裸鼠或经131I处理的免疫受损瑞士小鼠中的神经侵袭活性。结果表明，分枝杆菌HI - 75可能是瘰疬分枝杆菌的一个突变体，除了形成类麻风瘤病变外，还具有侵入周围神经的能力。