Bührer C, Luxenburger U, Metze B, Kattner E, Henze G, Dudenhausen J W, Obladen M
Department of Neonatology, Children's Hospital, Rudolf Virchow Medical Center, Berlin Free University, Germany.
Eur J Pediatr. 1993 Jun;152(6):519-22. doi: 10.1007/BF01955064.
L-Selectin, a leukocyte surface glycoprotein involved in white blood cell extravasation, is rapidly down-regulated after leukocyte activation. We prospectively determined lymphocyte L-selectin expression in freshly obtained cord blood samples of 98 neonates (gestational age 25-42 weeks). In eight infants with bacterial infection, the mean percentage of L-selectin(high) lymphocytes was 32.5% (SD 20.1%), compared to 60.1% (SD 18.7%) in the control group (P < 0.01). A percentage of L-selectin(high) lymphocytes of less than 42% had a sensitivity of 75% and a specificity of 82% in identifying infected newborns. Cord blood lymphocyte L-selectin expression was independent of gestational age, birth weight, umbilical artery pH, hematocrit, white blood cell count, absolute neutrophil count, C-reactive protein level, or maternal fever before delivery while there was a weak correlation with the newborn's immature/total ratio and platelet count. To our knowledge, this is the first report demonstrating downregulation of human lymphocyte L-selectin expression following activation of the immune system in vivo.
L-选择素是一种参与白细胞外渗的白细胞表面糖蛋白，在白细胞激活后会迅速下调。我们前瞻性地测定了98例新生儿（胎龄25 - 42周）新鲜采集的脐带血样本中淋巴细胞L-选择素的表达。在8例细菌感染婴儿中，L-选择素（高表达）淋巴细胞的平均百分比为32.5%（标准差20.1%），而对照组为60.1%（标准差18.7%）（P < 0.01）。L-选择素（高表达）淋巴细胞百分比低于42%在识别感染新生儿方面的敏感性为75%，特异性为82%。脐带血淋巴细胞L-选择素的表达与胎龄、出生体重、脐动脉pH值、血细胞比容、白细胞计数、绝对中性粒细胞计数、C反应蛋白水平或分娩前母亲发热无关，而与新生儿的未成熟/总比值和血小板计数存在弱相关性。据我们所知，这是第一份证明体内免疫系统激活后人淋巴细胞L-选择素表达下调的报告。
