Bührer C, Graulich J, Stibenz D, Dudenhausen J W, Obladen M
Departments of Neonatology, Children's Hospital, Berlin, Germany.
Pediatr Res. 1994 Dec;36(6):799-804. doi: 10.1203/00006450-199412000-00020.
The leukocyte glycoprotein L-selectin mediates an early step in the recruitment of leukocytes to sites of inflammation. L-Selectin surface expression is rapidly down-regulated by inflammatory signals in vitro. In a prospective study, we found L-selectin expression on umbilical cord blood granulocytes and monocytes to be significantly decreased in newborn infants with acute bacterial infection compared with controls (p < 0.01). A significantly reduced L-selectin expression of both granulocytes and monocytes was also found to be associated with an increased neutrophil immature/total ratio (p < 0.01) but not with other laboratory markers of neonatal sepsis. There was no apparent impact of prematurity, low birth weight, gestational hypertension, or gestational diabetes on L-selectin expression. Although the mode of delivery did not affect granulocyte L-selectin expression, umbilical cord blood monocytes showed an increased L-selectin expression after emergency cesarean delivery compared with samples obtained after elective cesarean or vaginal delivery (p < 0.01). We conclude that acute systemic inflammation results in down-regulation of granulocyte and monocyte L-selectin expression in vivo similar to that observed in vitro.
白细胞糖蛋白L-选择素介导白细胞募集至炎症部位的早期步骤。在体外，炎症信号可使L-选择素的表面表达迅速下调。在一项前瞻性研究中，我们发现与对照组相比，患有急性细菌感染的新生儿脐带血粒细胞和单核细胞上的L-选择素表达显著降低（p < 0.01）。还发现粒细胞和单核细胞的L-选择素表达显著降低与中性粒细胞未成熟/总数比值增加相关（p < 0.01），但与新生儿败血症的其他实验室指标无关。早产、低出生体重、妊娠期高血压或妊娠期糖尿病对L-选择素表达无明显影响。虽然分娩方式不影响粒细胞L-选择素表达，但与择期剖宫产或阴道分娩后获得的样本相比，急诊剖宫产后脐带血单核细胞的L-选择素表达增加（p < 0.01）。我们得出结论，急性全身性炎症导致体内粒细胞和单核细胞L-选择素表达下调，这与体外观察到的情况相似。
