Ortemann C, Robert F, Renaud B, Lambás-Señas L
Université Claude Bernard, Lyon, France.
J Neurochem. 1993 Aug;61(2):594-601. doi: 10.1111/j.1471-4159.1993.tb02163.x.
In vivo microdialysis coupled with HPLC and electrochemical detection was used to monitor extracellular levels of 3,4-dihydroxyphenylacetic acid (DOPAC) of the locus ceruleus (LC) in halothane-anesthetized rats. The identity of DOPAC was confirmed by experiments showing that the chromatographic peak was totally suppressed after inhibition of monoamine oxidase by pargyline. Histological examinations allowed to relate the quantity of DOPAC measured in the dialysates with the localization of the probe implantation site. We found that the DOPAC concentration was inversely proportional to the distance between the probe and the lateral border of the LC. Regardless of the caudorostral level of the nucleus, concentrations were maximal when the axis of the probe was 100 microns from the lateral border of the LC and decreased by 53% when this distance reached 300 microns. Activation of LC noradrenergic neurons by systemic administration of the alpha 2-antagonist piperoxane increased by 100% DOPAC concentrations in LC dialysates. These results suggest that the DOPAC measured by microdialysis could be considered an indicator of the functional state of LC noradrenergic neurons.
采用体内微透析结合高效液相色谱法和电化学检测法，监测氟烷麻醉大鼠蓝斑（LC）细胞外3,4 - 二羟基苯乙酸（DOPAC）水平。通过实验证实了DOPAC的身份，实验表明，在用优降宁抑制单胺氧化酶后，色谱峰完全被抑制。组织学检查使我们能够将透析液中测得的DOPAC量与探针植入部位的定位联系起来。我们发现，DOPAC浓度与探针和LC外侧边界之间的距离成反比。无论核的尾侧 - 头侧水平如何，当探针轴与LC外侧边界距离为100微米时，浓度最高，当该距离达到300微米时，浓度下降53%。通过全身给予α2拮抗剂哌克昔林激活LC去甲肾上腺素能神经元，可使LC透析液中DOPAC浓度增加100%。这些结果表明，通过微透析测得的DOPAC可被视为LC去甲肾上腺素能神经元功能状态的指标。
