Murata T, Ishibashi T, Inomata H
Department of Ophthalmology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ophthalmic Res. 1993;25(3):157-61. doi: 10.1159/000267284.
A consistent animal model of intraocular neovascularization was developed in the rat. A vitreous hemorrhage was induced by an experimental incision through the pars plana in the right eye of 24 rats. One month after the incision, 21 eyes produced a definite posthemorrhagic neovascularization arising from the optic disk. Histologically, the newly formed vessels consisted of endothelial cells forming lumina and surrounding smooth muscle cells. The proliferating activity of these cells was confirmed by immunohistochemical staining for proliferating cell nuclear antigen. An electron-microscopic examination also revealed mitotic activity and the cytoplasmic budding of immature endothelial cells. The 24 left eyes served as controls and showed no evidence of neovascularization.
在大鼠中建立了一种稳定的眼内新生血管动物模型。通过对24只大鼠右眼的睫状体扁平部进行实验性切口诱导玻璃体积血。切口后1个月，21只眼出现了明确的、源自视盘的出血后新生血管。组织学上，新形成的血管由形成管腔的内皮细胞和周围的平滑肌细胞组成。通过增殖细胞核抗原的免疫组织化学染色证实了这些细胞的增殖活性。电子显微镜检查也显示了未成熟内皮细胞的有丝分裂活性和细胞质出芽。24只左眼作为对照，未显示新生血管形成的迹象。
